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首页> 外文期刊>International journal of biomedical nanoscience and nanotechnology >Gemini nanoparticles as a co-delivery system for antigen - CpG oligodeoxynucleotide adjuvant combination
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Gemini nanoparticles as a co-delivery system for antigen - CpG oligodeoxynucleotide adjuvant combination

机译：Gemini纳米粒子作为抗原-CpG寡脱氧核苷酸佐剂组合的共同递送系统

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摘要

Enhancement of antigen-specific immune responses could be achieved by co-delivery of antigens and adjuvants. CpG oligodeoxynucleotides (unmethylated cytosine-guanine tandems in a specific sequence; ODNs) increase innate and antigen-specific immune responses. Effective co-delivery approaches of CpG ODNs and antigens to antigen-presenting cells are needed to achieve more potent antigen-specific immune responses. We evaluated both cellular and humoural immune responses triggered by hen egg lysozyme (HEL), a model antigen, and CpG ODNs formulated in gemini surfactant and dioleoyl phosphatidylcholine-based nanoparticles as an intradermal and topical co-delivery system in a murine animal model. Overall, intradermal injection of HEL/CpG nanoparticles induced a more pronounced Thl immune response compared with the HEL and HEL/CpG topical formulations, as evidenced by the shift in the Th2 response triggered by the antigen alone to a mixed Thl/Th2 immune response and increased the presence of interferon-gamma (IFN-gamma) secreting cells in the spleen. However, in case of topical administration, trie nanoparticle formulation of HEL produced enhanced immune response and immunomodulation even without the incorporation of CpG. The HEL-specific immune response and Thl bias demonstrated the advantage of co-delivery of HEL/CpG ODNs by gemini nanoparticles intradermally, whereas, the adjuvant effect of the nanoparticle delivery system itself was more significant after topical treatment.
机译：抗原和佐剂的共同递送可以实现抗原特异性免疫反应的增强。 CpG寡脱氧核苷酸（特定序列中未甲基化的胞嘧啶-鸟嘌呤单核苷酸; ODN）可增加先天和抗原特异性免疫应答。需要CpG ODN和抗原向抗原呈递细胞的有效共递送方法，以实现更有效的抗原特异性免疫反应。我们评估了由鸡蛋溶菌酶（HEL），模型抗原以及在双子表面活性剂和基于油酰磷脂酰胆碱的纳米颗粒中配制的CpG ODNs触发的细胞和体液免疫反应，作为鼠类动物模型中的皮内和局部共递送系统。总体而言，与HEL和HEL / CpG局部用药制剂相比，皮内注射HEL / CpG纳米颗粒可诱导更明显的Thl免疫应答，这可以通过单独的抗原触发的Th2应答向混合的Th1 / Th2免疫应答和增加了脾脏中干扰素-γ（IFN-γ）分泌细胞的存在。但是，在局部给药的情况下，即使没有掺入CpG，HEL的trie纳米颗粒制剂也能产生增强的免疫反应和免疫调节作用。 HEL特异性免疫反应和Th1偏倚证明了双子粒纳米粒子皮内共递送HEL / CpG ODN的优势，而局部治疗后，纳米粒子递送系统本身的佐剂作用更为显着。

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