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首页> 外文期刊>International journal of biomedical nanoscience and nanotechnology >Detection of environmental microorganisms with a bead-based assay and quantum dots
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Detection of environmental microorganisms with a bead-based assay and quantum dots

机译：通过基于珠的检测和量子点检测环境微生物

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Quantum dots (QDs) promise significant advantages in environmental and bead-based applications where the target cells need to be discriminated above background fluorescence. We examined the possible applications of QDs as markers for flow cytometric measurements. Paramagnetic beads were labelled with either QDs or some commonly-used fluorochromes and their fluorescence intensity was studied by flow cytometry after DNA capture or amplification. PCR amplicons were detected successfully when using a blue light excitation source for the QDs. Despite the low fluorescent signal of QDs compared to organic dyes found in this study, it is anticipated that the use of a UV laser instead of a blue laser would magnify the signal intensity significantly. We describe a bead-based method for identifying bacteria and archaea based on beads with an inherent fluorescent dye but applications of QDs may be reduced due to their large size relative to current fluorophores.
机译：量子点（QD）在环境和基于珠子的应用中具有显着的优势，在这些应用中，目标细胞需要在背景荧光之上进行区分。我们检查了QDs可能作为流式细胞仪测量的标志物的应用。用QD或一些常用的荧光染料标记顺磁珠，并在DNA捕获或扩增后通过流式细胞仪研究其荧光强度。将蓝光激发源用于QD时，PCR扩增子被成功检测到。尽管与本研究中发现的有机染料相比，量子点的荧光信号较低，但是可以预料的是，使用紫外线激光代替蓝色激光会显着放大信号强度。我们描述了一种基于珠子的方法，用于基于具有固有荧光染料的珠子来鉴定细菌和古细菌，但由于其相对于当前的荧光团较大，因此QD的应用可能会减少。

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