• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International journal of biomedical nanoscience and nanotechnology >Oligonucleotide-based signal amplification for ultrasensitive electrochemical biosensor
【24h】

Oligonucleotide-based signal amplification for ultrasensitive electrochemical biosensor

机译：基于寡核苷酸的信号放大，用于超灵敏电化学生物传感器

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

There is a need for an inexpensive, non-invasive and quick investigation with high sensitivity and specificity for early and accurate diagnosis of cancers. The present amplified bioassay takes advantage of the state-of-the-art electrical DNA detection methods, including the highly sensitive stripping response based on the intrinsic electroactivity of guanine and adenine nucleobases, and the amplification potential of latex microspheres carrying numerous oligonucleotide tags. The dramatic signal amplification advantage of this type of detection has been combined with efficient magnetic removal of non-complementary DNA for maximum selectivity, use of microlitre sample volumes and disposable transducers. The advantages of this procedure were demonstrated by its application in the detection of DNA segments related to the BRCA1 breast cancer gene.
机译：需要一种廉价，非侵入性且快速的研究，其具有高灵敏度和特异性以用于癌症的早期和准确诊断。本扩增的生物测定法利用了最先进的电DNA检测方法，包括基于鸟嘌呤和腺嘌呤核苷内在电活性的高度灵敏的剥离反应，以及带有众多寡核苷酸标签的乳胶微球的扩增潜力。这种检测方法具有显着的信号放大优势，并且可以有效地磁去除非互补DNA，以实现最大选择性，使用微升样品量和一次性传感器。该方法的优势已证明其在检测与BRCA1乳腺癌基因相关的DNA片段中的应用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号