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首页> 外文期刊>Indian Journal of Animal Nutrition >Calibration of In Vitro Gas Production Technique Using Digital Pressure Gauge: Air Versus Fermentative Gases
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Calibration of In Vitro Gas Production Technique Using Digital Pressure Gauge: Air Versus Fermentative Gases

机译：使用数字压力计校准体外气体生产技术：空气与发酵气体

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摘要

This experiment was aimed to compare air with fermentative gas for calibrating an in vitro gas production system using digital pressure gauge. Serum bottles (100 ml) containing 45 ml of in vitro medium were injected with air and fermentative gas produced from incubation of starch (S gas), starch and cellulose (S + C gas) and cellulose (C gas). The pressures in the bottles were read after equilibrating the vials overnight at 39 °C for each gas, calibration line was constructed by linear regression ofgauge reading (in psi) versus volume fraction (Fv) of added gas, where Fv = milliliters added gas/milliliters bottle headspace volume. For all four gases (air, S..gas, S + C gas and C gas), there was a linear relationship between volume and pressure (r~2 = 0.99). There was no significant difference between slopes of calibration curve obtained from air, S gas, S + C gas and C gas. Results of present work indicated that calibration of in vitro gas production system using air can give reliable regressionequation applicable for measurement of total gas volume of wide ranges of substrates.
机译：该实验旨在比较空气和发酵气体，以使用数字压力表校准体外气体生产系统。向装有45 ml体外培养基的血清瓶（100 ml）注入空气和通过发酵淀粉（S气体），淀粉和纤维素（S + C气体）和纤维素（C气体）产生的发酵气体。在每种温度下将小瓶在39°C下平衡一夜后，读取瓶中的压力，通过量规读数（以psi为单位）对添加气体的体积分数（Fv）进行线性回归来构建校准线，其中Fv =毫升添加气体/毫升瓶顶空体积。对于所有四种气体（空气，S..gas，S + C气体和C气体），体积与压力之间存在线性关系（r〜2 = 0.99）。从空气，S气体，S + C气体和C气体获得的校准曲线的斜率之间没有显着差异。当前工作的结果表明，使用空气对体外气体生产系统进行校准可以提供可靠的回归方程，适用于测量各种基材的总气体体积。

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