...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International journal of fertility and women's medicine >Cryopreservation of human spermatozoa: comparison of TEST-yolk buffer and glycerol.
【24h】

Cryopreservation of human spermatozoa: comparison of TEST-yolk buffer and glycerol.

机译：人类精子的冷冻保存：TEST卵黄缓冲液和甘油的比较。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

OBJECTIVE: Comparison of human sperm motility, morphology, and sperm membrane integrity using two cryoprotective media, Test-yolk buffer with glycerol and glycerol alone. METHODS: Semen samples from 10 healthy donors were divided and frozen with either glycerol or a combination of glycerol and TEST-yolk buffer. Semen characteristics were evaluated before freezing and after thawing. Motility was measured at 0, 60, 120, and 180 minutes post-thaw following removal of the cryoprotectant (post-wash). RESULTS: Percentage of motile sperm decreased significantly compared to prefreeze values in both groups. Post-thaw motility following removal of the freezing media was higher in specimens that were frozen in TEST-yolk buffer compared to those frozen in glycerol at 0 minutes (P = 0.004). Similarly, specimens cryopreserved in TEST-yolk buffer had higher sperm motility compared to aliquots that were frozen in glycerol at 180 minutes (P = 0.013). The percentage of normal sperm forms was significantly higher post-wash and post-thaw in specimens that were cryopreserved in TEST-yolk buffer (P = 0.04). CONCLUSIONS: Sperm cryopreserved in TEST-yolk buffer had significantly better motility, morphology, and sperm membrane integrity than sperm preserved in glycerol alone.
机译：目的：使用两种冷冻保护介质，卵黄质缓冲液（仅含甘油和甘油）比较人精子的运动力，形态和精子膜的完整性。方法：将来自10个健康供体的精液样品分开，并用甘油或甘油和TEST卵黄缓冲液的组合冷冻。冷冻前和解冻后评估精液特性。在除去冷冻保护剂后（解冻后），在解冻后0、60、120和180分钟测量运动性。结果：与预冻值相比，两组的运动精子百分比均显着下降。与在0分钟时在甘油中冷冻的样品相比，在TEST蛋黄缓冲液中冷冻的样品中去除冷冻介质后的解冻后运动性更高。同样，冷冻保存在TEST卵黄缓冲液中的标本与在180分钟的甘油中冷冻的等分试样相比，具有更高的精子活力（P = 0.013）。在TEST蛋黄缓冲液中冷冻保存的标本中，正常精子形式的百分比在洗后和解冻后明显更高（P = 0.04）。结论：冷冻保存在TEST蛋黄缓冲液中的精子比单独保存甘油的精子具有更好的运动性，形态和精子膜完整性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号