• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Horticulture & Crop Science Research >In vitro Organogenesis and Somatic Embryogenesis in Adenia hondala (Gaertn.) de Wilde, an Endangered Medicinal Plant of the Western Ghats.
【24h】

In vitro Organogenesis and Somatic Embryogenesis in Adenia hondala (Gaertn.) de Wilde, an Endangered Medicinal Plant of the Western Ghats.

机译：西高止山脉濒危药用植物本田埃德尼亚（Gaertn.de Wilde）的体外器官发生和体细胞胚发生。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

An efficient and rapid regeneration protocol was developed from nodal explants and callus cultures of Adenia hondala on Murashige and Skoog's (MS) medium supplemented with BAP and Kn. The highest number of shoots (8.25 ± 0.2) from nodal explants wereobtained at an optimum combination of 0.5 mg l~(-1) BAP + 0.5mg l~(-1)Kn + 10 % CW. The highest shoot elongation (8.25 ±0.6 cms) was recorded at a concentration of 0.5 mg l~(-1) BAP + 0.5mg 1~(-1)2 ip. Compact green callus was initiated from young internodal explants of MS medium supplemented with 3 mg l~(-1) 2, 4 - D + 1mg l~(-1) BAP. Organogenesis was achieved by transferring callus on MS medium supplemented with 1 mg L"1 BAP + 0.5 mg L"1 Kn. Highest number of shoots 14.25 ± 1.5 shoots / culture were initiated as clusters on the callus. In vitro regenerated shoots were then excised from shoot clumps and transferred to rooting medium containing half strength MS medium.Somatic embryogenesis was obtained directly from leaf explants at a concentration of 2 mg l~(-1) NAA + 0.5 mg l~(-1) BAP + 200 mg l~(-1) CH. 34 ± 0.2 embryos / culture were produced. Indirect somatic embryogenesis from friable calli was induced in 28 days at the optimum concentration of 1 mg l~(-1) NAA + 0.5 mg l~(-1) BAP + 0.5 mg l~(-1) TDZ. The highest number of embryos 62.4 ± 2.5 per culture was produced at this concentration. After 15 days of culture globular embryoids became heart shaped and thentorpedo shaped. The elongated embryoids showed distinct bipolar growth forming a shoot pole and root pole. The rooted plantlets were hardened on liquid half MS medium and subsequently in polycups containing sterile soilrite. Plantlets thus developed were successfully established and transferred to a green house. The plantlets showed survival rate of 90 % in soil. Biochemical estimation of total protein,starch and soluble sugars when carried out in different stages of in vitro cultures indicated metabolic mobilization during organogenesis.
机译：通过在补充BAP和Kn的Murashige和Skoog's（MS）培养基上从本外腺的结节外植体和愈伤组织培养物中开发出一种高效，快速的再生方案。在0.5 mg l〜（-1）BAP + 0.5mg l〜（-1）Kn + 10％CW的最佳组合下，从结节外植体中获得了最高的芽数（8.25±0.2）。在0.5 mg l〜（-1）BAP + 0.5mg 1〜（-1）2 ip的浓度下记录到最高的枝条伸长率（8.25±0.6 cms）。致密的绿色愈伤组织是由补充3 mg l〜（-1）2、4-D + 1mg l〜（-1）BAP的MS培养基的年轻节间外植体引发的。通过将愈伤组织转移到补充有1 mg L“ 1 BAP + 0.5 mg L” 1 Kn的MS培养基上来实现器官发生。在愈伤组织上以丛生形式开始最高芽数14.25±1.5芽/培养。然后从枝条上切下离体的再生芽并转移到含半强度MS培养基的生根培养基中，直接从叶片外植体中以2 mg l〜（-1）NAA + 0.5 mg l〜（-1）的浓度获得体细胞胚发生。 BAP + 200 mg l〜（-1）CH。产生34±0.2个胚胎/培养物。在最适浓度为1 mg l〜（-1）NAA + 0.5 mg l〜（-1）BAP + 0.5 mg l〜（-1）TDZ的情况下，在28天之内诱导了脆性愈伤组织的间接体细胞胚发生。在此浓度下，每个培养物中产生的胚数最高，为62.4±2.5。培养15天后，球形胚状体变成心形，然后变成鱼雷形。细长的类胚表现出明显的双极生长，形成芽极和根极。将生根的小苗在液体半MS培养基上硬化，然后在含有无菌土的聚杯中硬化。这样开发的小植株成功建立并转移到温室中。幼苗在土壤中的存活率为90％。在体外培养的不同阶段进行的总蛋白质，淀粉和可溶性糖的生化估计表明器官发生期间代谢动员。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号