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首页> 外文期刊>International journal of developmental neuroscience: the official journal of the International Society for Developmental Neuroscience >Expression of vascular endothelial growth factor (VEGF) mRNA, VEGF receptor 2 (Flk-1) mRNA, and of VEGF co-receptor neuropilin (Nrp)-1 mRNA in brain tissue of aging Tg2576 mice by in situ hybridization
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Expression of vascular endothelial growth factor (VEGF) mRNA, VEGF receptor 2 (Flk-1) mRNA, and of VEGF co-receptor neuropilin (Nrp)-1 mRNA in brain tissue of aging Tg2576 mice by in situ hybridization

机译：原位杂交技术在衰老Tg2576小鼠脑组织中表达血管内皮生长因子（VEGF）mRNA，VEGF受体2（Flk-1）mRNA和VEGF共受体神经毛蛋白（Nrp）-1 mRNA

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Vascular endothelial growth factor (VEGF) has been characterized as a heparin binding angiogenic growth factor displaying high specificity for endothelial cells. It is profoundly accumulated and co-localized with amyloid beta (A beta) plaques in the brain of Alzheimer's disease patients. In order to examine the effect of AS plaques on the expression level of VEGF mRNA and its receptors, brain tissue of both transgenic Tg2576 and wild type mice at ages ranging from 13 to 22 months was subjected to in situ hybridization followed by densitometric assessment using computer-assisted image analysis. Strong expression of VEGF mRNA, fetal liver kinase (Flk)-1 mRNA, and neuropilin (Nrp)-1 mRNA in the piriform, entorhinal, somatosensory, frontal cortex and hippocampal formation of both transgenic and non-transgenic mice brain was detected. Developmentally, only expression of VEGF mRNA was increased with age in the entorhinal, and somatosensory cortex of wild type mice. In 20-month-old transgenic Tg2576 mice, upregulation of VEGF mRNA, Flk-1 mRNA, and Nrp-1 mRNA transcripts was observed in the entorhinal cortex compared to age-matched wild type mice. Our data suggest up-regulation of VEGF mRNA, Flk-1 mRNA and Nrp-1 mRNA, at least in the entorhinal cortex at ages when AS deposition in Tg2576 is typically increasing. (C) 2015 Elsevier Ltd. All rights reserved.
机译：血管内皮生长因子（VEGF）已被表征为肝素结合血管生成生长因子，对内皮细胞具有高度特异性。它在阿尔茨海默氏病患者的大脑中深度积累并与淀粉样蛋白β（A beta）斑块共定位。为了检查AS斑对VEGF mRNA及其受体表达水平的影响，对13至22个月大的转基因Tg2576和野生型小鼠的脑组织进行原位杂交，然后使用计算机进行光密度评估辅助图像分析。检测到在转基因和非转基因小鼠大脑的梨状，内嗅，体感，额叶皮层和海马形成中VEGF mRNA，胎儿肝激酶（Flk）-1 mRNA和神经纤维蛋白（Nrp）-1 mRNA的强表达。在发育上，野生型小鼠的内嗅和体感皮层中只有VEGF mRNA的表达随年龄增加。在20个月大的转基因Tg2576小鼠中，与年龄匹配的野生型小鼠相比，在内嗅皮层中观察到VEGF mRNA，Flk-1 mRNA和Nrp-1 mRNA转录的上调。我们的数据表明，至少在内脏皮质中，在Tg2576中AS沉积通常增加的年龄，VEGF mRNA，Flk-1 mRNA和Nrp-1 mRNA的表达上调。 （C）2015 Elsevier Ltd.保留所有权利。

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