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首页> 外文期刊>Biochemical Genetics >Morphological and genetic variations of the freshwater leech, Hirudinaria spp., in Peninsular Malaysia
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Morphological and genetic variations of the freshwater leech, Hirudinaria spp., in Peninsular Malaysia

机译：马来西亚半岛淡水le Hirudinaria spp。的形态和遗传变异

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摘要

In this study the genetic diversity of local freshwater leeches (Hirudinaria spp.) was inferred using mtDNA COI gene analysis and compared with the gross external variations of 26 freshwater leech specimens obtained from the wild and leech farms. Based on a neighbor-joining tree generated from 516 COI base sequences, four distinct clades of Hirudinaria were seen with interspecific genetic divergence in the range of 7.6-14.5%. The external morphological variations based on the presence of stripes, location of gonopores, and anus separated the samples into four morphologically distinct groups matching the four clades obtained from the molecular data. Two black stripes at the ventral region were observed only in specimens found clustered with clades that contained the GenBank-reported H. manillensis, whereas the brown or dark green coloration without stripes on the ventral region was seen in samples that clustered with H. javanica and H. bpling clades.
机译：在这项研究中，使用mtDNA COI基因分析推断了当地淡水水ches（Hirudinaria spp。）的遗传多样性，并与从野生和水ech养殖场获得的26个淡水水le标本的总体外部变化进行了比较。基于从516个COI碱基序列产生的邻居连接树，观察到四个不同的Hirudinaria进化枝，其种间遗传差异在7.6-14.5％的范围内。基于条纹的存在，线虫孔的位置和肛门的外部形态变化将样品分为四个形态上不同的组，这些组与从分子数据中获得的四个进化枝相匹配。仅在与包含GenBank报道的H. manillensis的进化枝成簇的标本中观察到在腹侧区域有两个黑色条纹，而在与爪哇木和H. javanica聚类的样品中，在腹侧区域看到棕色或深绿色的无条纹H. bpling进化枝。

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