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首页> 外文期刊>British journal of ophthalmology >Immunolocalisation of E-cadherin and beta-catenin in human pterygium.
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Immunolocalisation of E-cadherin and beta-catenin in human pterygium.

机译：E-钙粘蛋白和β-连环蛋白在人翼状ery肉中的免疫定位。

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AIM: The pterygium represents an invasion of a wing of altered ocular surface tissue into the normal cornea. The head itself is slightly elevated and white, which is the only site of firm adhesion to the globe. The mechanisms of cell proliferation and adhesion in pterygium epithelium, however, are unknown. The aim of this study was to analyse the expression of cell adhesion molecules in pterygium tissues. METHODS: Six pterygia were surgically removed using the bare-sclera procedure, and two normal corneas and a normal bulbar conjunctiva were also obtained. Formalin-fixed, paraffin-embedded tissues were analysed by immunohistochemistry with anti-E-cadherin and beta-catenin antibodies. RESULTS: Immunoreactivity for E-cadherin was not detected in the normal cornea and conjunctiva. In contrast, all corneal and conjunctival epithelial cells showed a weak homogeneous immunoreaction for beta-catenin on the cell membrane. In the pterygium head, the thickness was relatively marked compared with the body, and normal conjunctival and corneal epithelia. E-cadherin as well as beta-catenin was heterogeneously expressed in the cell membrane and cytoplasm of a variety of epithelial cells, whereas the expression was less marked in the body. Several epithelial cells showed intense nuclear immunoreactivity for beta-catenin. Immunoreactivity of beta-catenin, but not E-cadherin, was detected in only a few stromal cells, which were less marked than in epithelial cells. CONCLUSION: It is suggested that E-cadherin and beta-catenin are concentrated in pterygium tissue, and are possibly involved with epithelial proliferation and adhesion.
机译：目的：翼状represents肉代表眼表组织改变的翅膀侵入正常角膜。头部本身略微升高，呈白色，这是牢固粘附到地球上的唯一部位。然而，翼状ery肉上皮细胞增殖和粘附的机制尚不清楚。这项研究的目的是分析翼状ery肉组织中细胞粘附分子的表达。方法：采用裸眼巩膜手术切除6例翼状ery肉，并获得2例正常角膜和1例正常球结膜。用抗E-钙粘蛋白和β-连环蛋白抗体通过免疫组织化学分析福尔马林固定，石蜡包埋的组织。结果：在正常角膜和结膜中未检测到E-钙粘蛋白的免疫反应性。相反，所有角膜和结膜上皮细胞对细胞膜上的β-catenin均显示弱的均质免疫反应。在翼状head肉头中，与身体以及正常结膜和角膜上皮相比，厚度相对明显。 E-钙粘蛋白和β-连环蛋白在多种上皮细胞的细胞膜和细胞质中异质表达，而在体内的表达较少。几个上皮细胞对β-catenin表现出强烈的核免疫反应性。仅在少数基质细胞中检测到了β-catenin的免疫反应性，但未检测到E-cadherin的免疫反应性，而这些细胞的标记性不如上皮细胞。结论：E-钙粘蛋白和β-连环蛋白集中在翼状tissue肉组织中，可能与上皮细胞的增殖和粘附有关。

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