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首页> 外文期刊>International journal of immunogenetics >The molecular characterization of a catalase from Chinese mitten crab Eriocheir sinensis.
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The molecular characterization of a catalase from Chinese mitten crab Eriocheir sinensis.

机译：中国中华绒螯蟹中华绒螯蟹过氧化氢酶的分子表征。

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摘要

Catalase (CAT) is an antioxidant enzyme and plays a significant role in the protection against oxidative stress by reducing hydrogen peroxide. The CAT cDNA of Eriocheir sinensis (EsCAT) was cloned via RACE technique. The complete sequence of EsCAT cDNA consisted of a 5' untranslated regions (UTR) of 224 bp, a 3' UTR of 1287 bp with a poly (A) tail and an open reading frame (ORF) of 1542 bp, which encoded a polypeptide of 513 amino acid residues with a calculated molecular mass of approximately 58.86 kDa and a theoretical isoelectric point of 6.880. The deduced amino acid sequence of EsCAT contained a highly conserved proximal active-site signature motif ((60)FDRERIPERVVHAKGAL(76)) and a proximal heme-ligand signature motif ((350)RLFSYNDTH(358)) and exhibited high similarity with other reported CATs. In the phylogenetic tree, EsCAT was clustered with the CATs from Scylla serrata and Portunus trituberculatus. The EsCAT transcripts were constitutively expressed in haepatopancreas, haemocytes, gill, gonad, muscle and heart, with highest expression level in haepatopancreas. The relative expression level of EsCAT mRNA in haemocytes was continuously up-regulated and reached the peak level at 48 h post-Vibrio anguillarum challenge. The purified recombinant EsCAT protein displayed antioxidant activity against hydrogen peroxide with high thermal stability and broad spectrum of pH values. All these results demonstrated that EsCAT was an efficient antioxidant enzyme and potentially involved in the regulation of redox and innate immune response of crabs.
机译：过氧化氢酶（CAT）是一种抗氧化酶，在通过减少过氧化氢来抵抗氧化应激的保护中起着重要作用。通过RACE技术克隆了中华绒螯蟹的CAT cDNA（EsCAT）。 EsCAT cDNA的完整序列由224 bp的5'非翻译区（UTR），1287 bp的3'UTR（带有多聚（A）尾巴）和1542 bp的开放阅读框（ORF）组成，后者编码多肽513个氨基酸残基，计算分子量约为58.86 kDa，理论等电点为6.880。推导的EsCAT氨基酸序列包含高度保守的近端活性部位签名基序（（60）FDRERIPERVVHAKGAL（76））和近端血红素配体签名基序（（350）RLFSYNDTH（358）），并且与其他报道的氨基酸具有高度相似性猫。在系统发育树中，EsCAT与来自Scyla serrata和Portunus trituberculatus的CAT聚集在一起。 EsCAT转录本在肝胰腺，血细胞，g，性腺，肌肉和心脏中组成性表达，在肝胰腺中表达水平最高。 EsCAT mRNA在血细胞中的相对表达水平持续上调，并在鳗弧菌激发后48小时达到峰值。纯化的重组EsCAT蛋白显示出对过氧化氢的抗氧化活性，具有高的热稳定性和宽广的pH值范围。所有这些结果表明，EsCAT是一种有效的抗氧化酶，可能参与了蟹的氧化还原和先天免疫反应的调节。

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