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首页> 外文期刊>Biochemical Genetics >RNF187 is Downregulated Following NF-kappa B Inhibition in Late Erythroblasts
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RNF187 is Downregulated Following NF-kappa B Inhibition in Late Erythroblasts

机译：晚期成红细胞中NF-κB抑制后，RNF187被下调

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摘要

Beta (beta)-thalassaemic erythroblasts grown in vitro have reduced nuclear factor kappa B (NF-kappa B) pathway gene expression. By inhibiting this pathway in erythroblasts from normal individuals, important downstream genes affected by this inhibition can be identified. Bay 11-7082 is a potent inhibitor of the NF-kappa B pathway, it acts irreversibly, inhibiting NF-kappa B activation by blocking tumor necrosis factor alpha (TNF-alpha)-induced phosphorylation of the inhibitory I kappa B subunit thereby preventing NF-kappa B activation. In this study, hematopoietic stem cells were isolated from the peripheral blood of 6 healthy individuals and were then cultured for 14 days in conditions which promote erythroid differentiation. Following erythroid lineage enrichment, these cells were stimulated with TNF alpha or inhibited with Bay 11-7082. Subsequent RNA isolation and gene expression analyses were performed using pooled cDNA with custom PCR arrays. Genes of interest were examined individually on non-pooled samples. Our data identified RNF187, a RING finger domain gene as being downregulated in response to NF-kappa B inhibition.
机译：体外生长的β（β）地中海贫血成红细胞具有减少的核因子κB（NF-κB）途径基因表达。通过抑制来自正常个体的成红细胞中的该途径，可以鉴定受该抑制作用影响的重要下游基因。 Bay 11-7082是NF-κB通路的有效抑制剂，它不可逆地发挥作用，通过阻断肿瘤坏死因子α（TNF-alpha）诱导的抑制性IκB亚基的磷酸化来抑制NF-κB活化。 -k B激活。在这项研究中，从6名健康个体的外周血中分离出造血干细胞，然后在促进类红细胞分化的条件下培养14天。红细胞谱系富集后，这些细胞用TNFα刺激或用Bay 11-7082抑制。随后的RNA分离和基因表达分析使用带有定制PCR阵列的合并cDNA进行。在非合并样本中单独检查目标基因。我们的数据确定了RING指域基因RNF187在响应NF-κB抑制时被下调。

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