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首页> 外文期刊>British journal of ophthalmology >Diagnostic approaches for oculoglandular tularemia: advantages of PCR.
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Diagnostic approaches for oculoglandular tularemia: advantages of PCR.

机译：眼睑妥拉血症的诊断方法：PCR的优势。

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摘要

AIM: The authors describe a diagnostic approach that proved to be particularly valuable in rare cases of ocular tularemia registered during the tularemia outbreak in 1997-2005 in Bulgaria. The authors describe the laboratory findings and diagnosis of four cases with an oculoglandular form of infection. METHODS: Several different specimens from each patient were analysed. Oculoglandular tularemia was diagnosed in four patients either by culture, immunofluorescent antibody analysis (IFA), serology or by a polymerase chain reaction (PCR) assay. Results and DISCUSSION: Three F tularensis strains were isolated and characterised. One of these was isolated from a conjuctival swab specimen obtained from a seronegative patient. The authors report for the first time a successful application of diagnostic PCR performed directly on conjuctival swab specimen. From all analysed specimens IFA was diagnostically effective only in the case of lymph node aspirates and was not sensitive enough for conjuctival swabs or blood samples. The authors also describe the histological picture of a conjunctival granuloma in course of infection. All patients were successfully treated with ciprofloxacin. CONCLUSIONS: Some of the proposed laboratory diagnostic strategies (swab PCR) are not invasive and could represent a new approach for resolving rare and hard-to-diagnose cases of oculoglandular tularemia.
机译：目的：作者描述了一种诊断方法，该方法被证明对保加利亚在1997-2005年暴动性暴发期间登记的罕见眼动性暴动特别有用。作者描述了实验室检查结果和四例眼睑形式感染的诊断。方法：对每位患者的几种不同标本进行了分析。通过培养，免疫荧光抗体分析（IFA），血清学或通过聚合酶链反应（PCR）分析诊断出四名患者的眼眶小肠性血肿。结果与讨论：分离并鉴定了3株F tularensis菌株。其中之一是从血清阴性患者的结膜拭子样本中分离出来的。作者首次报告直接在结膜拭子样本上成功进行了诊断PCR的成功应用。从所有分析过的标本中，IFA仅在淋巴结抽吸的情况下才具有诊断效力，并且对结膜拭子或血液样本不够敏感。作者还描述了感染过程中结膜肉芽肿的组织学图片。所有患者均成功用环丙沙星治疗。结论：某些拟议的实验室诊断策略（拭子PCR）不是侵入性的，可能代表了一种解决罕见和难诊断性眼睑妥拉血病的新方法。

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