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首页> 外文期刊>International journal of immunogenetics >Molecular cloning and sequencing of MHC class II beta 1 domain of turkey reveals high sequence identity with chicken.
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Molecular cloning and sequencing of MHC class II beta 1 domain of turkey reveals high sequence identity with chicken.

机译：土耳其MHC II类β1结构域的分子克隆和测序揭示了与鸡的高度序列同一性。

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摘要

We report the nucleotide sequences of turkey (Meleagris gallopavo) major histocompatibility complex (MHC) class II loci (beta 1 domain or exon 2 encoding the peptide-binding region). In the present investigation, three distinct sequences from the beta 1 domain of turkey MHC class II were isolated. A BLAST search and phylogenetic analysis revealed that turkey MHC sequences are most similar to chicken and peacock MHC. There was no strong evidence of recombination among the turkey MHC sequences or with other avian MHC, but diversity was high. The diversity in this peptide-binding region may be the result of point mutation and balancing selection or frequent gene conversion within turkey. However, more work and data are needed to understand the evolution of turkey and other avian MHC. Moreover, polymerase chain reaction-restriction fragment-length polymorphism analysis of exon 2 using the Hinf I restriction enzyme demonstrated three restriction patterns and a preliminary evidence of multiple beta loci in turkey. PCR-RFLP analysis of turkey MHC class II loci could be a promising method of MHC genotyping, when more sequences are available. Turkey MHC haplotypes identified earlier by RFLP analysis should be sequenced to standardize turkey MHC nomenclature and to develop DNA based method of haplotyping.
机译：我们报告了土耳其（Meleagris gallopavo）主要组织相容性复合体（MHC）II类位点（β1域或编码肽结合区的外显子2）的核苷酸序列。在本研究中，从土耳其MHC II类的beta 1域中分离了三个不同的序列。 BLAST搜索和系统发育分析表明，火鸡MHC序列与鸡和孔雀MHC最相似。没有强有力的证据表明土耳其MHC序列之间或与其他禽类MHC发生重组，但多样性很高。这种肽结合区的多样性可能是点突变和平衡选择的结果，或者是土耳其内部频繁的基因转换的结果。但是，需要更多的工作和数据来了解土耳其和其他禽类MHC的进化。此外，使用Hinf I限制性内切酶对外显子2的聚合酶链反应-限制性片段长度多态性分析显示了三种限制性模式和火鸡中多个β基因座的初步证据。当有更多序列可用时，对土耳其MHC II类基因座进行PCR-RFLP分析可能是一种有前途的MHC基因分型方法。早期通过RFLP分析鉴定的土耳其MHC单倍型应进行测序，以标准化土耳其MHC命名法，并开发基于DNA的单倍型方法。

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