...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International journal of immunogenetics >Molecular cloning and expression of a Relish gene in Chinese mitten crab Eriocheir sinensis.
【24h】

Molecular cloning and expression of a Relish gene in Chinese mitten crab Eriocheir sinensis.

机译：中华绒螯蟹中华绒螯蟹Relish基因的分子克隆与表达。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

NF-kappaB is a B-cell specific transcription factor that plays crucial roles in inflammation, immunity, apoptosis, development and differentiation. In the present study, a novel NF-kappaB-like transcription factor Relish was cloned from Chinese mitten crab Eriocheir sinensis (designated as EsRelish) by rapid amplification of cDNA ends (RACE) technique based on expressed sequence tag (EST). The full-length cDNA of EsRelish was of 5034 bp, consisting of a 5' untranslated region (UTR) of 57 bp, a 3' UTR of 1335 bp with two mRNA instability motifs (ATTTA), a polyadenylation signal sequence (AATAAA) and a poly (A) tail, and an open reading frame (ORF) of 3645 bp encoding a polypeptide of 1214 amino acids with a calculated molecular mass of 134.8 kDa and a theoretical isoelectric point of 5.26. There were a typical Rel homology domain (RHD), two nuclear localization signal (NLS) sequences (KR), an inhibitor kappaB (IkappaB)-like domain with six ankyrin repeats, a PEST region and a death domain in the deduced amino acid sequence of EsRelish. Conserved domain, higher similarity with other Rel/NF-kappaBs and phylogenetic analysis suggested that EsRelish was a member of the NF-kappaB family. Quantitative real-time RT-PCR was employed to detect the mRNA transcripts of EsRelish in different tissues and its temporal expression in hemocytes of E. sinensis challenged with Pichia methanolica and Listonella anguillarum. The EsRelish mRNA was found to be constitutively expressed in a wide range of tissues. It could be mainly detected in the hemocytes, gonad and hepatopancreas, and less degree in the gill, muscle and heart. The expression level of EsRelish mRNA in hemocytes was up-regulated from at 3, 6, 9 and 12 h after P. methanolica challenge. In L. anguillarum challenge, it was up-regulated at 9, 12 and 24 h. The results collectively indicated that EsRelish was potentially involved in the immune response against fungus and bacteria.
机译：NF-κB是B细胞特异性转录因子，在炎症，免疫，细胞凋亡，发育和分化中起关键作用。本研究通过基于表达序列标签（EST）的cDNA末端快速扩增（RACE）技术，从中华绒螯蟹（Eriocheir sinensis，命名为EsRelish）克隆了一种新型的NF-κB样转录因子Relish。 EsRelish的全长cDNA为5034 bp，由57 bp的5'非翻译区（UTR），1335 bp的3'UTR和两个mRNA不稳定基序（ATTTA），聚腺苷酸化信号序列（AATAAA）和聚（A）尾，和3645 bp的开放阅读框（ORF），其编码1214个氨基酸的多肽，计算的分子量为134.8 kDa，理论等电点为5.26。推导的氨基酸序列中有一个典型的Rel同源结构域（RHD），两个核定位信号（NLS）序列（KR），一个抑制剂kappaB（IkappaB）样结构域，具有六个锚蛋白重复序列​​，一个PEST区和一个死亡结构域。 EsRelish。保守域，与其他Rel / NF-kappaB的相似性更高以及系统发育分析表明EsRelish是NF-kappaB家族的成员。采用定量实时RT-PCR检测不同组织中EsRelish的mRNA转录本，以及在甲醇毕赤酵母和安氏李斯特菌攻击下的中华绒螯蟹血细胞中的瞬时表达。发现EsRelish mRNA在多种组织中组成性表达。它主要在血细胞，性腺和肝胰腺中检出，在degree，肌肉和心脏中检出的程度较低。甲醇毕赤酵母攻击后3、6、9和12小时，血细胞中EsRelish mRNA的表达水平上调。在安圭拉菌的挑战中，它在9、12和24小时被上调。结果共同表明，EsRelish可能参与了针对真菌和细菌的免疫反应。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号