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首页> 外文期刊>International journal of immunogenetics >Cytokine gene polymorphism in Iranian patients with chronic myelogenous leukaemia.
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Cytokine gene polymorphism in Iranian patients with chronic myelogenous leukaemia.

机译：伊朗慢性粒细胞性白血病患者细胞因子基因多态性。

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摘要

Chronic myelogenous leukaemia (CML) is a disorder of the haematopoietic stem cell that results in malignant expansion of myeloid cells with a cytogenetic abnormality, and translocation between chromosomes 9 and 22, known as the Philadelphia chromosome. It has been hypothesized that genetic factors other than histocompatibility disparity may play a role in predisposition to developing CML. In this regard, T helper types 1 and 2 (Th1 and Th2) cytokines and their gene polymorphism seem to be important. Overall expression and secretion of cytokines are dependent, at least in part, on genetic polymorphism (nucleotide variations) within the promoter region or other regulatory sequences of cytokine genes. The majority of polymorphisms described are single nucleotide polymorphism (SNPs). The objective of this study was to analyse the genetic profile of Th1 and Th2 cytokines in 30 Iranian patients with CML and 40 healthy subjects. In the patients and control subjects, the allelic and genotype frequencies were determined for the cytokine genes. All typing were performed with a polymerase chain reaction-sequence-specific primers (PCR-SSP) assay. Allele and genotype frequencies were calculated and compared with those of normal controls. The results showed that the most frequent genotypes in our patients were transforming growth factor (TGF)-beta TG/TG, interferon (IFN)-gamma AT, interleukin (IL)-4 CC at position -590, TT at position -33, and IL-10 ACC/ACC and ATA/ATA. In contrast, the genotypes TGF-beta CG/CG, IL-2 TT at position -330, IL-4 CT at position -590, CT at position -33, and IL-10 GCC/ACC were seen at much lower frequencies. The results suggest that production of TGF-beta in CML patients is higher and production of IL-4 and IL-10 is lower than in normal subjects.
机译：慢性粒细胞性白血病（CML）是造血干细胞的一种疾病，会导致髓样细胞发生恶性扩张，并伴有细胞遗传学异常，并在9号和22号染色体之间移位，这就是费城染色体。据推测，除了组织相容性差异外，遗传因素可能在诱发CML的易感性中起作用。在这方面，T辅助细胞1型和2型（Th1和Th2）细胞因子及其基因多态性似乎很重要。细胞因子的总体表达和分泌至少部分取决于细胞因子基因的启动子区域或其他调控序列内的遗传多态性（核苷酸变异）。所描述的大多数多态性是单核苷酸多态性（SNP）。这项研究的目的是分析30名伊朗CML患者和40名健康受试者的Th1和Th2细胞因子的遗传特征。在患者和对照组中，确定了细胞因子基因的等位基因和基因型频率。所有类型均使用聚合酶链反应序列特异性引物（PCR-SSP）分析进行。计算等位基因和基因型频率，并与正常对照者比较。结果显示，我们患者中最常见的基因型是转化生长因子（TGF）-βTG / TG，干扰素（IFN）-γAT，白介素（IL）-4 CC位于-590位，TT位于-33位， IL-10 ACC / ACC和ATA / ATA。相反，在较低的频率下可以看到基因型TGF-βCG / CG，-330处的IL-2 TT，-590处的IL-4 CT，-33处的CT和IL-10 GCC / ACC。结果表明，与正常人相比，CML患者中TGF-β的产生较高，而IL-4和IL-10的产生较低。

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