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首页> 外文期刊>Biochemical Genetics >Camelus dromedarius putative cytochrome P450 enzyme CYP2E1: complete coding sequence and phylogenetic tree.
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Camelus dromedarius putative cytochrome P450 enzyme CYP2E1: complete coding sequence and phylogenetic tree.

机译:驼峰推定细胞色素P450酶CYP2E1:完整的编码序列和系统发育树。

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摘要

This study determined the full-length sequence of CYP2E1, one of six cytochrome P450 genes previously examined in camel tissues by western blotting and semi-quantitative PCR. The Camelus dromedarius CYP2E1 has an open reading frame of 1,473 bp, and the cDNA encodes a protein of 490 amino acid residues with a molecular weight of 54.8 kDa. The deduced amino acid sequence showed the highest identity with Bos taurus (88%), Sus scrofa (87%), and Homo sapiens (83%). In a phylogenetic analysis, the C. dromedarius CYP2E1 isoform was located beside cattle and pigs. The deduced amino acid sequence of camel CYP2E1 showed the conserved proline-rich amino terminus and the heme-binding signature localized near the carboxy terminus of the protein.
机译:这项研究确定了CYP2E1的全长序列,这是先前通过蛋白质印迹和半定量PCR在骆驼组织中检查过的六个细胞色素P450基因之一。 Camelus dromedarius CYP2E1的开放阅读框为1,473 bp,cDNA编码一个490个氨基酸残基的蛋白质,分子量为54.8 kDa。推导的氨基酸序列显示出与金牛座(88%),南方鳄(87%)和智人(83%)的同一性最高。在系统发育分析中,dromedarius CYP2E1亚型位于牛和猪的旁边。骆驼CYP2E1的推导氨基酸序列显示保守的富含脯氨酸的氨基末端和血红素结合标记位于蛋白质的羧基末端附近。

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