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首页> 外文期刊>Biochemical Genetics >Comparative assessment of genetic variability in the populations of endemic and endangered yellow catfish, Horabagrus brachysoma (Teleostei: Horabagridae), based on allozyme, RAPD, and microsatellite markers.
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Comparative assessment of genetic variability in the populations of endemic and endangered yellow catfish, Horabagrus brachysoma (Teleostei: Horabagridae), based on allozyme, RAPD, and microsatellite markers.

机译：根据同工酶，RAPD和微卫星标记，对地方性和濒危黄色cat鱼（Horabagrus brachysoma，Teleostei：Horabagridae）种群的遗传变异进行比较评估。

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摘要

The comparative assessment of genetic diversity using allozymes, random amplified polymorphic DNA (RAPD), and microsatellite markers was conducted in endemic and endangered yellow catfish (Horabagrus brachysoma) sampled from three locations in Western Ghats river systems of India. Among the three markers, microsatellites show more polymorphism, having 100% polymorphic loci, whereas allozymes show the least (56%). In RAPD, 60.5% of fragments were polymorphic. Observed heterozygosity and F(ST) values were very high in microsatellites, compared with the other markers. Microsatellite and RAPD markers reported a higher degree of genetic differentiation than allozymes among the populations depicted by pairwise F(ST)/G(ST), AMOVA, Nei's genetic distance, and UPGMA dendrogram. The three classes of markers demonstrated striking genetic differentiation between pairs of H. brachysoma populations. The data emphasize the need for fishery management, conservation, and rehabilitation of this species.
机译：在印度西高止山脉河系三个地点采样的地方性和濒危黄色cat鱼（Horabagrus brachysoma）中，使用同工酶，随机扩增多态性DNA（RAPD）和微卫星标记对遗传多样性进行了比较评估。在这三个标记中，微卫星显示出更多的多态性，具有100％的多态性位点，而同工酶显示得最少（56％）。在RAPD中，有60.5％的片段是多态的。与其他标记相比，微卫星中观察到的杂合度和F（ST）值非常高。在成对的F（ST）/ G（ST），AMOVA，Nei的遗传距离和UPGMA树状图所描绘的人群中，微卫星和RAPD标记的遗传分化程度高于同工酶。这三类标记物证明了成对的短臂嗜血杆菌种群之间具有惊人的遗传分化。数据强调了该物种的渔业管理，保护和恢复的需要。

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