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首页> 外文期刊>International journal of medical microbiology: IJMM >Distribution of Rickettsia helvetica in Ixodes ricinus tick populations in Poland
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Distribution of Rickettsia helvetica in Ixodes ricinus tick populations in Poland

机译：波兰立克次氏tick壁虱种群中立克次体的分布

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摘要

Questing Ixodes ricinus ticks from different regions of Poland were investigated for the presence of spotted fever group (SFG) rickettsiae. A total of 1214 DNA samples of 2813 ticks, including 820 individual adult ticks and 394 pools containing 1993 nymphs, were tested by PCR for a fragment of the rickettsial gltA gene using the primers RpCs.877 and RpCs.1258. Overall, at least 5.5% ticks were found to be positive with the highest prevalence observed in females (10.6%). A sample of 14 positive PCR products was sequenced. Analyzed fragments of 270-370 bp showed 100% similarities to corresponding sequences of Rickettsia helvetica deposited in the GenBank. Results of our investigation confirm the occurrence and wide distribution of R. helvetica in L ricinus tick populations in Poland. This rickettsia should be added to the list of potentially dangerous pathogens transmitted by ticks in our country. (C) 2008 Elsevier GrnbH. All rights reserved.
机译：调查了来自波兰不同地区的大鳍x（Rixinus ricinus）s，调查了发现的立克次体发烧组（SFG）立克次体。使用引物RpCs.877和RpCs.1258，通过PCR检测了立克次体gltA基因的片段，对总共1214个2813个壁虱的DNA样本，包括820个成年的壁虱和394个包含1993年若虫的库进行了测试。总体而言，发现至少5.5％的s虫是阳性的，而在女性中观察到的患病率最高（10.6％）。对14种阳性PCR产物的样品进行了测序。 270-370 bp的分析片段显示与GenBank中存放的立克次体的相应序列的100％相似性。我们的调查结果证实了在波兰的蓖麻油虱tick虫种群中Helvetica的发生和广泛分布。该立克次氏体应添加到我国tick虫传播的潜在危险病原体列表中。 （C）2008 Elsevier GrnbH。版权所有。

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