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首页> 外文期刊>International journal of legal medicine >Development of SNP-based human identification system.
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Development of SNP-based human identification system.

机译:基于SNP的人类身份识别系统的开发。

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摘要

Single nucleotide polymorphisms (SNPs) appeal to the forensic DNA community because of their abundance in the human genome, low mutation rate, small amplicon size, and feasibility of high-throughput genotyping technologies.In an initial screening, we identified six SNP markers of sex determination by resequencing the amelogenin genes and the zinc finger protein genes located on the sex chromosomes. Furthermore, for use in human identification,we selected 30 highly polymorphic autosomal SNP markers from among a human population and examined the potential utility of these SNP markers for human identification.The combined mean match probability of 30 SNP markers was 4.83 x 10(-13). Using genotyping data from 8,842 unrelated Korean individuals, we also found that discrimination power increased 10-fold for the addition of every five SNP markers in human identification. In this study, we demonstrated that SNP markers are very useful for sex determination and human identification, even in a very homogeneous population.
机译:单核苷酸多态性(SNP)由于其在人类基因组中的丰富性,低突变率,小的扩增子大小以及高通量基因分型技术的可行性而吸引了法医DNA社区。在初步筛选中,我们鉴定了六个SNP性标志物通过对位于性染色体上的牙釉蛋白基因和锌指蛋白基因进行重测序来确定。此外,为了用于人类识别,我们从人群中选择了30种高度多态性常染色体SNP标记,并研究了这些SNP标记在人类识别中的潜在用途.30个SNP标记的组合平均匹配概率为4.83 x 10(-13 )。使用来自8,842名韩国无关亲戚的基因分型数据,我们还发现,在人类识别中每增加五个SNP标记,识别力就会增加10倍。在这项研究中,我们证明了即使在非常同质的人群中,SNP标记对于性别确定和人类识别也非常有用。

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