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首页> 外文期刊>International journal of medical microbiology: IJMM >VTEC O157 subtypes associated with the most severe clinical symptoms in humans constitute a minor part of VTEC 0157 isolates from Danish Cattle
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VTEC O157 subtypes associated with the most severe clinical symptoms in humans constitute a minor part of VTEC 0157 isolates from Danish Cattle

机译：与人类最严重的临床症状相关的VTEC O157亚型仅占丹麦牛VTEC 0157分离株的一小部分

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The aim of this study was to compare the distribution of VTEC 0157 subtypes isolated from human sporadic infections with those in the Danish bovine reservoir, and to correlate the subtypes with the severity of the clinical symptoms in humans. The study included a total of 149 Danish eae-positive VTEC 0 157 isolates (63 of bovine origin and 86 from human clinical cases) isolated between 1987 and 2001. All were analysed by vtx-PCR-RFLP and phage typing. The vtx-PCR-RFLP showed that isolates carrying the vtx2 gene was more than four times as prevalent among the human clinical isolates (55%) as compared to the bovine isolates (13%). Furthermore, a significant correlation between the presence of the vtx2 gene and development of haemolytic-uraemic syndrome was found. The 149 isolates encompassed 16 different phage types (PTs). The majority (87%) of the human clinical isolates were identified, as PT2, PT4, PT8 or PT14 while only 46% of the bovine isolates belonged to these PTs. PT8 and PT14 were found at similar rates among bovine (36%) and human isolates (40%). However, the predominant PTs in the human isolates, PT2 (19%) and PT4 (28%), were only identified in 2% and 8%, respectively, of the bovine isolates. All but one PT2 and PT4 isolate carried either vtx2 alone or in combination with vtx2c, whereas none of the PT8 and PT14 isolates carried vtx2. The significant overlap between vtx/phage type combinations in bovine and human clinical isolates indicate that cattle are an important reservoir for human VTEC 0157 infections in Denmark. However, the vtx2-carrying isolates, causing the most severe clinical symptoms, constitute only a minor fraction of the isolates from the Danish bovine reservoir. (C) 2004 Elsevier GmbH. All rights reserved.
机译：这项研究的目的是比较从人零星感染中分离出的VTEC 0157亚型与丹麦牛库中的VTEC 0157亚型的分布，并将这些亚型与人类临床症状的严重程度相关联。该研究共纳入了1987年至2001年之间分离的149株丹麦eae阳性VTEC菌株（来自牛的63例，来自人类临床病例的86例）。所有样本均通过vtx-PCR-RFLP和噬菌体分型进行了分析。 vtx-PCR-RFLP显示，与牛分离株（13％）相比，携带vtx2基因的分离株在人类临床分离株（55％）中的流行率是其四倍以上。此外，发现vtx2基因的存在与溶血尿毒症综合征的发展之间存在显着相关性。 149个分离株涵盖16种不同的噬菌体类型（PTs）。鉴定出人类临床分离物中的大多数（87％）为PT2，PT4，PT8或PT14，而只有46％的牛分离物属于这些PT。在牛（36％）和人分离株（40％）中发现PT8和PT14的比率相似。但是，在人类分离株中，主要的PTs PT2（19％）和PT4（28％）分别仅在2％和8％的牛分离株中被鉴定。除一个PT2和PT4分离株外，所有分离株均单独携带vtx2或与vtx2c结合使用，而PT8和PT14分离株均不携带vtx2。牛和人类临床分离物中的vtx /噬菌体类型组合之间的显着重叠表明，牛是丹麦人类VTEC 0157感染的重要储存库。但是，携带vtx2的分离株会引起最严重的临床症状，仅占丹麦牛库中分离株的一小部分。 （C）2004 Elsevier GmbH。版权所有。

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