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首页> 外文期刊>International journal of medical microbiology: IJMM >CsrA and CsrB are required for the post-transcriptional control of thevirulence-associated effector protein AvrA of Salmonella enterica
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CsrA and CsrB are required for the post-transcriptional control of thevirulence-associated effector protein AvrA of Salmonella enterica

机译：转录后控制肠炎沙门氏菌的病毒相关效应蛋白AvrA所需的CsrA和CsrB

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摘要

The virulence-associated effector protein AvrA of Salmonella enterica is an ubiquitin-like acetyltransferase/cysteineprotease, which interferes with the first line of immune response of the target organism. In contrast to translation of theAvrA protein in S. enterica strains, which takes place either constitutively (class 1 strains), or after acid induction (class2 strains), or not at all (class 3 strains); the constitutive transcription of the respective avrA genes occurs regardless ofthese defined expression classes. When the number of avrA genes and mRNA molecules is raised experimentally usingplasmids carrying the respective cloned avrA genes together with their promoter regions, the translation of avrAmRNA takes place very strongly in all respective AvrA expression classes. This kind of copy-dependent, post-transcriptional control of AvrA was shown to be dependent on the regulatory action of the CsrA/CsrB system since thedeletion of both genes completely abolished the translation in the tested S. enterica strains, whereas the transcriptionremained unaffected. Moreover, AvrA production in strains carrying the cloned avrA genes on plasmids remaineddependent on the presence of CsrA but unaffected in csrB mutant strains. On the other hand, overproduction of theregulatory molecules CsrA and CsrB in S. enterica strains carrying cloned csrA and csrB genes on plasmids ceased theexpression of AvrA again. Therefore, the expression of avrA is suggested to be regulated in a post-transcriptionalmanner by critical and effective concentrations of CsrA (see-saw regulation), which is achieved through thesequestering activity of CsrB.
机译：肠炎沙门氏菌的毒力相关效应蛋白AvrA是一种泛素样乙酰基转移酶/半胱氨酸蛋白酶，可干扰靶标生物的第一线免疫反应。与AvrA蛋白在肠炎链球菌菌株中的翻译相反，后者是组成型（1类菌株）或在酸诱导后（2类菌株）或根本不发生（3类菌株）;不论这些定义的表达类别如何，均会发生相应的avrA基因的组成性转录。当使用携带各自克隆的avrA基因及其启动子区域的质粒实验性提高avrA基因和mRNA分子的数量时，avrAmRNA的翻译在所有各自的AvrA表达类型中都非常强烈地发生。由于对这两个基因的删除完全消除了被测试的肠炎链球菌菌株的翻译，AvrA的这种依赖拷贝的转录后控制被证明依赖于CsrA / CsrB系统的调节作用。而且，在质粒上携带克隆的avrA基因的菌株中AvrA的产生仍然取决于CsrA的存在，但在csrB突变菌株中不受影响。另一方面，在质粒上携带克隆的csrA和csrB基因的肠炎链球菌菌株中调节分子CsrA和CsrB的过量生产再次终止了AvrA的表达。因此，建议通过关键和有效浓度的CsrA（跷跷板调节）在转录后方式调节avrA的表达，这是通过CsrB的这些活性来实现的。

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