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首页> 外文期刊>International journal of legal medicine >First experiences using the new Powerplex(R) ESX17 and ESI17 kits in casework analysis and allele frequencies for two different regions in Germany.
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First experiences using the new Powerplex(R) ESX17 and ESI17 kits in casework analysis and allele frequencies for two different regions in Germany.

机译：在德国两个不同地区进行案例分析和等位基因频率分析时，使用新的Powerplex®ESX17和ESI17套件的初次体验。

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DNA databases are the most efficient tools in criminal investigations with unknown perpetrators. Due to a significant number of random matches in cross-border DNA profile exchanges, the European Network of Forensic Science Institutes (ENFSI) proposed the addition of further short tandem repeats (STRs) to European DNA databases. Therefore, the new Powerplex(R) ESX17 and Powerplex(R) ESI17 kits from Promega comprised the 11 established DNA database STRs and additionally the well-known loci D1S1656 and D12S391, as well as D2S441, D10S1248, and D22S1045. The latter three STRs are thereby established as so-called mini-STRs to fulfill the increasing requirements regarding sensitivity and reproducibility for analysis of minute amounts of DNA. Here, we provide allele frequencies for the five additional STRs from two populations from Germany. A test regarding suitability and robustness of the new kits for routine trace analysis showed that it is more likely to obtain a meaningful profile using Powerplex(R) ESX17 and Powerplex(R) ESI17 kits compared to the Powerplex(R) ES kit. However, for both new kits the range of template DNA amount is rather small, e.g., slightly more DNA than recommended resulted in DNA profiles which could not be reliably evaluated due to allelic drop-in or imbalances and overshoots. In our opinion, the new kits are very promising new tools in forensic trace analysis even though handling and evaluation should yet be carried out with great caution.
机译：DNA数据库是对不明犯罪者进行刑事调查时最有效的工具。由于跨界DNA配置文件交换中存在大量随机匹配项，因此，欧洲法医科学研究所网络（ENFSI）建议在欧洲DNA数据库中添加更多的短串联重复序列（STR）。因此，来自Promega的新的Powerplex ESX17和Powerplex ESI17试剂盒包括11个已建立的DNA数据库STR以及另外的众所周知的基因座D1S1656和D12S391，以及D2S441，D10S1248和D22S1045。因此，后三个STR被建立为所谓的微型STR，以满足对微量DNA分析的灵敏度和可重复性不断增长的要求。在这里，我们提供了来自德国两个人群的五个额外STR的等位基因频率。关于新试剂盒用于常规痕量分析的适用性和耐用性的测试表明，与Powerplex®ES试剂盒相比，使用Powerplex®ESX17和Powerplex®ESI17试剂盒更有可能获得有意义的概况。但是，对于这两种新试剂盒，模板DNA量的范围都相当小，例如，比推荐量多一些的DNA会导致DNA谱图，由于等位基因插入或不平衡和过冲，无法可靠地评估DNA谱图。我们认为，即使应该非常谨慎地进行处理和评估，新试剂盒还是法医痕量分析中非常有前途的新工具。

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