首页> 外文期刊>International journal of legal medicine >Identification of forensically important Sarcophaga species (Diptera: Sarcophagidae) using the mitochondrial COI gene.
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Identification of forensically important Sarcophaga species (Diptera: Sarcophagidae) using the mitochondrial COI gene.

机译:使用线粒体COI基因鉴定法医上重要的石棺物种(双翅目:石棺科)。

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摘要

The identification of species of the forensically important genus Sarcophaga is very difficult and requires strong taxonomic expertise. In this study, we sequenced the mitochondrial cytochrome c oxidase subunit I (COI) gene of 126 specimens of 56?W European Sarcophaga species and added GenBank data to our database to yield a total dataset of 270 COI sequences from 99 Sarcophaga species to evaluate the COI gene as a molecular diagnostic tool for species identification in this genus. Using two simple criteria (Best Match, BM and Best Close Match, BCM), we showed that the identification success using a mini-barcode region of 127?bp was very low (80.7-82.5?%) and the use of this region is not recommended as a species identifier. In contrast, identification success was very high using the standard barcode region (658?bp) or using the entire COI region (1,535?bp) (98.2-99.3?%). Yet, there was a low interspecific sequence divergence (<2?%) in six species groups so that for 16 out of the 99 species (nine of which are of forensic importance), the use of COI barcodes as species identifier should be done with care. For these species, additional markers will be necessary to achieve a 100?% identification success. We further illustrate how such reference databases can improve local reference databases for forensic entomologists.
机译:鉴定具有法医学重要性的石棺属的物种非常困难,需要强大的分类学专业知识。在这项研究中,我们对56个W欧洲石棺物种的126个标本的线粒体细胞色素C氧化酶I(COI)基因进行了测序,并将GenBank数据添加到我们的数据库中,以产生来自99种石棺物种的270个COI序列的总数据集,以评估COI基因作为该属物种鉴定的分子诊断工具。使用两个简单的标准(最佳匹配,BM和最佳接近匹配,BCM),我们显示使用127bp的迷你条形码区域进行识别的成功率非常低(80.7-82.5%),并且使用不建议用作物种标识符。相反,使用标准条形码区域(658?bp)或使用整个COI区(1,535?bp)(98.2-99.3%),识别成功率很高。然而,六个物种组之间的种间序列差异很低(<2%),因此对于99个物种中的16个物种(其中9个具有法医学意义),应使用COI条形码作为物种标识符关心。对于这些物种,为了获得100%的识别成功率,将需要使用其他标记。我们将进一步说明此类参考数据库如何改善法医昆虫学家的本地参考数据库。

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