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首页> 外文期刊>International journal of mass spectrometry >Electron capture dissociation and collision induced dissociation behavior of peptides containing methionine, selenomethionine and oxidized methionine
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Electron capture dissociation and collision induced dissociation behavior of peptides containing methionine, selenomethionine and oxidized methionine

机译:包含蛋氨酸,硒代蛋氨酸和氧化蛋氨酸的肽的电子俘获解离和碰撞诱导解离行为

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摘要

The electron capture dissociation and collision induced dissociation of doubly and triply charged ions of peptides RPLGMSPFK, RPLGM(Se)SPFK and RPLGM(0)SPFK were determined by Fourier transform ion cyclotron resonance mass spectrometry. The results suggest that ECD of analyzed species strongly depends on the nature of a single element (i.e., sulfur and selenium atoms, sulfoxide group) and can provide both primary sequence information and structural information regarding the modification. The properties of sulfur and selenium atoms are negligible in the case of CID processes. The CID loss of CH3SOH, which is unique in peptides to the Met(O) side chain, does not depend on the peptide sequence. (C) 2015 Elsevier B.V. All rights reserved.
机译:通过傅立叶变换离子回旋共振质谱法确定了肽RPLGMSPFK,RPLGM(Se)SPFK和RPLGM(0)SPFK的双电荷和三电荷离子的电子俘获解离和碰撞诱导解离。结果表明,所分析物种的ECD在很大程度上取决于单个元素的性质(即,硫和硒原子,亚砜基),并且可以提供有关修饰的主要序列信息和结构信息。在CID工艺中,硫和硒原子的特性可以忽略不计。 CH3SOH的CID丢失在肽中对于Met(O)侧链是唯一的,它不取决于肽序列。 (C)2015 Elsevier B.V.保留所有权利。

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