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首页> 外文期刊>International journal of molecular medicine >Validation of three splice donor and three splice acceptor sites for regulating four novel low-abundance spliced transcripts of human cytomegalovirus UL21.5 gene locus
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Validation of three splice donor and three splice acceptor sites for regulating four novel low-abundance spliced transcripts of human cytomegalovirus UL21.5 gene locus

机译：验证三个剪接供体和三个剪接受体位点，以调节人巨细胞病毒UL21.5基因位点的四个新的低丰度剪接转录本

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摘要

In a previous study, one spliced transcript of human cytomegalovirus (HCMV), named UL21.5 was identified. UL21.5 has been found to be one of the viral transcripts packaged within HCMV particles. The UL21.5 mRNA is translated into a secreted glycoprotein, which is a viral chemokine decoy receptor specifically interacting with regulated upon activation normal T cell expressed and secreted (RANTES). In the present study, four novel low-abundance 3-coterminal spliced transcripts were identified to be transcribed from the UL21.5 gene region of a low-passage HCMV strain during the late infection phase by cDNA library screening, northern blot hybridization, reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE)-PCR. Three splicing donor and three splicing acceptor sites found in the UL21.5 gene region were validated to be functional in an in vitro expression system. In addition, the determinant regulatory region that is necessary for the splice donor site at nucleotide (nt) 25533 was located in a 9-bp sequence around the site; the regulatory regions for the splice acceptor sites at nt 26597 and nt 26633 were located in a 20-bp sequence upstream of the site at nt 26597 and in a 10-bp sequence from nt 26641 to nt 26650 downstream of the site at nt 26633, respectively.
机译：在先前的研究中，鉴定了人类巨细胞病毒（HCMV）的一个剪接转录本，命名为UL21.5。已发现UL21.5是包装在HCMV颗粒内的病毒转录物之一。 UL21.5 mRNA被翻译成一种分泌的糖蛋白，它是一种病毒趋化因子诱饵受体，与激活的正常T细胞表达和分泌的调节因子特异性相互作用（RANTES）。在本研究中，通过cDNA文库筛选，northern blot杂交，反转录，在感染后期，从低通量HCMV株的UL21.5基因区域中转录出4个新的低丰度3共末端剪接转录本。聚合酶链反应（RT-PCR）和cDNA末端快速扩增（RACE）-PCR。在UL21.5基因区域中发现的三个剪接供体和三个剪接受体位点已在体外表达系统中得到验证。另外，核苷酸（nt）25533的剪接供体位点所必需的决定性调控区位于该位点周围的9 bp序列中。 nt 26597和nt 26633的剪接受体位点的调控区位于nt 26597的位点上游20 bp序列中，nt 26633的位点下游从nt 26641到nt 26650的10 bp序列中，分别。

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