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首页> 外文期刊>International journal of molecular medicine >microRNA-214 enhances the invasion ability of breast cancer cells by targeting p53
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microRNA-214 enhances the invasion ability of breast cancer cells by targeting p53

机译：microRNA-214通过靶向p53增强乳腺癌细胞的侵袭能力

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摘要

Breast cancer (BC) is the foremost cause of cancer-related mortality in women worldwide. An increasing number of studies has confirmed that microRNAs (miRNAs or miRs) play an important role in the development and progression of BC. microRNA-214 (miR-214), a member of the miRNA family, has been demonstrated to function as both a tumor suppressor and oncogene in various types of human cancer. However, the biological function of miR-214 in BC remains unclear. The present study was designed to investigate the potential role of miR-214 in the development and progression of BC. Our results revealed that miR-214 expression was significantly increased in the BC tissues compared with the adjacent benign tissues, and that the upregulation of miR-214 was significantly associated with the invasion ability of the BC cells. Furthermore, p53, which has been reported to be downregulated in BC, was predicted to be the target gene of miR-214 using bioinformatics software programs. Moreover, luciferase reporter vectors were constructed and it was confirmed that p53 is a target of miR-214. Following the transfection of miR-214 into BC cells, we found that the overexpression of miR-214 markedly enhanced cell invasion through the downregulation of p53 expression. By contrast, the overexpression of p53 abrogated the effects of miR-214. In conclusion, this study demonstrates that miR-214 functions as an oncogene in BC, at least partly by promoting cell invasion through the downregulation of p53. Therefore, miR-214 may be a potential therapeutic target for the treatment of BC.
机译：乳腺癌（BC）是全世界女性与癌症相关的死亡率的最主要原因。越来越多的研究证实，microRNA（miRNA或miRs）在BC的发展和进程中起着重要的作用。 miRNA家族的一个成员microRNA-214（miR-214）已被证明在多种类型的人类癌症中既具有抑癌作用，又具有致癌基因的功能。但是，miR-214在BC中的生物学功能仍不清楚。本研究旨在调查miR-214在BC的发展和进程中的潜在作用。我们的结果表明，与邻近的良性组织相比，miR-214在BC组织中的表达明显增加，并且miR-214的上调与BC细胞的侵袭能力显着相关。此外，据报道，使用生物信息学软件程序，已在BC中下调了p53，它是miR-214的靶基因。此外，构建了荧光素酶报告载体，并证实p53是miR-214的靶标。将miR-214转染至BC细胞后，我们发现miR-214的过表达通过下调p53表达显着增强了细胞侵袭。相反，p53的过表达消除了miR-214的作用。总之，这项研究表明，miR-214在BC中起癌基因的作用，至少部分是通过下调p53促进细胞入侵而实现的。因此，miR-214可能是治疗BC的潜在治疗靶标。

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