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首页> 外文期刊>International journal of molecular medicine >REIC/Dkk-3 stable transfection reduces the malignant phenotype of mouse prostate cancer RM9 cells.
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REIC/Dkk-3 stable transfection reduces the malignant phenotype of mouse prostate cancer RM9 cells.

机译：REIC / Dkk-3稳定转染可降低小鼠前列腺癌RM9细胞的恶性表型。

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摘要

The reduced expression in immortalized cells (REIC)/Dickkopf (Dkk)-3, a member of the Dkk gene family, is a tumor suppressor in a broad range of cancers. REIC/Dkk-3 transfected stable clones of mouse prostate cancer RM9 cells (RM9-REIC) and the empty vector-transfected control clone cells (RM9-EV) were established. Clones were used to evaluate the anti-cancer effects and a proteomics analysis of REIC/Dkk-3 continuous expression was performed. The RM9-REIC cells show a feeble appearance and the cell membrane shows irregular buds known as blebs. In vitro cell proliferation was significantly suppressed in RM9-REIC clones in comparison to the control. The apoptosis assay was done under standard culture conditions and RM9-REIC showed a higher incidence of apoptosis. The RM9-EV and RM9-REIC cells were orthotopically implanted into a C57BL/6 mouse prostate. After 2 weeks, the tumor growth was significantly inhibited in RM9-REIC cells in comparison to the control. Two-dimensional gel electrophoresis was used to examine the modification of protein expression by the gene transfection. The analysis with mass spectrometry disclosed that expression of peroxiredoxin-1, GST-P1, transgelin-2, MRP-L12, ARD, GRP78 and Sorcin were increased and eEF1A-1 and cyclophilin-40 protein were decreased in RM9-REIC cells. Therefore, REIC/Dkk-3 stable transfectants show a reduction of malignancy in mouse prostate cancer RM9 cells in vitro and in vivo. The result of the proteomics analysis might provide important clues to clarify the anti-cancer molecular mechanism of REIC/Dkk-3 gene transfer.
机译：Dkk基因家族成员永生化细胞（REIC）/ Dickkopf（Dkk）-3中的表达降低是多种癌症中的肿瘤抑制因子。建立了REIC / Dkk-3转染的小鼠前列腺癌RM9细胞的稳定克隆（RM9-REIC）和空载体转染的对照克隆细胞（RM9-EV）。使用克隆评估抗癌作用，并进行了REIC / Dkk-3连续表达的蛋白质组学分析。 RM9-REIC细胞显示出微弱的外观，细胞膜显示出不规则的芽，称为起泡。与对照相比，RM9-REIC克隆中的体外细胞增殖被显着抑制。在标准培养条件下进行凋亡测定，并且RM9-REIC显示出更高的凋亡发生率。将RM9-EV和RM9-REIC细胞原位植入C57BL / 6小鼠前列腺中。 2周后，与对照相比，RM9-REIC细胞中的肿瘤生长被显着抑制。二维凝胶电泳用于检查基因转染对蛋白质表达的修饰。质谱分析表明，在RM9-REIC细胞中，peroxiredoxin-1，GST-P1，transgelin-2，MRP-L12，ARD，GRP78和Sorcin的表达增加，而eEF1A-1和cyclophilin-40的蛋白质减少。因此，REIC / Dkk-3稳定转染子在体外和体内显示小鼠前列腺癌RM9细胞的恶性程度降低。蛋白质组学分析的结果可能为阐明REIC / Dkk-3基因转移的抗癌分子机制提供重要线索。

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