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首页> 外文期刊>International journal of molecular medicine >(-)-Epigallocatechin gallate attenuates the induction of HSP27 stimulated by sphingosine 1-phosphate via suppression of phosphatidylinositol 3-kinase/Akt pathway in osteoblasts.
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(-)-Epigallocatechin gallate attenuates the induction of HSP27 stimulated by sphingosine 1-phosphate via suppression of phosphatidylinositol 3-kinase/Akt pathway in osteoblasts.

机译：（-）-表没食子儿茶素没食子酸酯通过抑制成骨细胞中磷脂酰肌醇3-激酶/ Akt途径减弱了鞘氨醇1-磷酸刺激的HSP27诱导。

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摘要

We previously reported that sphingosine 1-phosphate stimulates the induction of heat shock protein 27 (HSP27) through p38 mitogen-activated protein (MAP) kinase and phosphatidylinositol 3-kinase/Akt in osteoblast-like MC3T3-E1 cells. In the present study, we investigated whether (-)-epigallocatechin gallate (EGCG), the major polyphenol found in green tea, affects the induction of HSP27 in these cells and its mechanism, since it was previously reported that catechin, including EGCG, suppresses bone resorption. EGCG significantly reduced the induction of HSP27 stimulated by sphingosine 1-phosphate in a dose-dependent manner between 10 and 30 microM. Immunofluorescence microscopy studies revealed that sphingosine 1-phosphate certainly stimulated the induction of HSP27 in the cytosol of these cells, and that EGCG clearly suppressed its induction. However, sphingosine 1-phosphate-stimulated phosphorylation of p38 MAP kinase or MAPKAP-2 was not affected by EGCG. By contrast, EGCG markedly suppressed the phosphorylations of both Akt and glycogen synthase kinase-3beta stimulated by sphingosine 1-phosphate. These results strongly suggest that EGCG suppresses the induction of HSP27 stimulated by sphingosine 1-phosphate via attenuation of, not the p38 MAP kinase pathway, but of the phosphatidylinositol 3-kinase/Akt pathway in osteoblasts.
机译：我们以前报道过，1-磷酸鞘氨醇通过成骨细胞样MC3T3-E1细胞中的p38丝裂原活化蛋白（MAP）激酶和磷脂酰肌醇3-激酶/ Akt刺激热休克蛋白27（HSP27）的诱导。在本研究中，我们调查了绿茶中发现的主要多酚（-）-表没食子儿茶素没食子酸酯（EGCG）是否会影响HSP27在这些细胞中的诱导作用及其机制，因为先前已报道儿茶素（包括EGCG）会抑制骨吸收。 EGCG以剂量依赖性的方式在10至30 microM之间显着降低了鞘氨醇1-磷酸酯刺激的HSP27诱导。免疫荧光显微镜研究表明，1-磷酸鞘氨醇无疑刺激了这些细胞的细胞质中HSP27的诱导，而EGCG明显抑制了其诱导。但是，EGCG不影响鞘氨醇1-磷酸刺激的p38 MAP激酶或MAPKAP-2的磷酸化。相反，EGCG明显抑制了鞘氨醇1-磷酸酯刺激的Akt和糖原合酶激酶3β的磷酸化。这些结果强烈提示，EGCG通过减缓成骨细胞中的p38 MAP激酶途径而非磷脂酰肌醇3激酶/ Akt途径，抑制了鞘氨醇1-磷酸刺激的HSP27诱导。

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