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首页> 外文期刊>International journal of PIXE >Application of micro-pixe camera to elemental analysis of a single cell
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Application of micro-pixe camera to elemental analysis of a single cell

机译：微型摄像机在单细胞元素分析中的应用

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Since the cellular metabolism has not yet been fully elucidated, it is very useful to get the information of elemental distributions in a single cell. We applied a micro-PIXE camera to obtain a picture presented with density of an element in a cell. Our micro-PIXE camera is on the basis of PIXE analysis using micro ion beams with the spot size of less than 1 #mu#m and the samples can be analyzed in air, so the size and the state of samples are not limited. Here, we analyzed the spatial distributions of elements in a single cell of animal for a medical application. Bovine aortic endothelial cells (BAECs) were cultured in culture medium containing bromodeoxyuridine (BrdU) which is known to be used in the DNA synthesis. We could take the elemental maps of phosphorus, potassium and sulfur which present well a shape of cell and a position of nucleus in this cell. Moreover, we obtained the picture of Br, that is, BrdU. It was found that BrdU was locally concentrated in two or three regions in the nucleus.
机译：由于细胞代谢尚未完全阐明，因此获得单个细胞中元素分布的信息非常有用。我们应用了微型PIXE相机来获取带有单元中元素密度的图像。我们的微型PIXE相机是基于PIXE分析的，它使用的光斑尺寸小于1＃mu＃m，并且可以在空气中分析样品，因此样品的尺寸和状态不受限制。在这里，我们分析了用于医学应用的动物单个细胞中元素的空间分布。牛主动脉内皮细胞（BAEC）在含有溴脱氧尿苷（BrdU）的培养基中培养，该溴脱氧尿苷已知用于DNA合成。我们可以获取磷，钾和硫的元素图，这些元素图很好地显示了细胞的形状和细胞中核的位置。此外，我们获得了Br的图片，即BrdU。发现BrdU局部集中在细胞核的两个或三个区域。

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