...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International journal of PIXE >SAMPLE PREPARATION METHOD USING A MICROTOME FOR BIOLOGICAL SPECIMENS
【24h】

SAMPLE PREPARATION METHOD USING A MICROTOME FOR BIOLOGICAL SPECIMENS

机译：使用微生物标本的样品制备方法

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The preparation of a biological sample using a microtome cryostat was attempted. The biological specimen was previously frozen with a freezing compound and an optimum section thickness was cut for PIXE analysis. First, bovine liver powder which was made into a paste with indium solution as an internal standard was prepared using the sectioning method. The concentrations of trace elements including volatile elements in the sample were determined and agreed with the certified values. Next, a mouse liver was prepared using the sectioning method. A polyphenylene sulfide (PPS) film containing a constant areal density of sulfur was used as a backing material for quantitative analysis. The concentrations of trace elements in a mouse liver prepared using the sectioning method were compared with those using the ordinary nitric acid method. The concentrations of some volatile elements were determined only in the sample obtained using the sectioning method. The preparation method using a microtome cryostat can be used to prepare a biological sample for PIXE analysis.
机译：尝试使用切片机低温恒温器制备生物样品。预先用冷冻化合物冷冻生物样品，并切出最佳切片厚度以进行PIXE分析。首先，使用切片法制备以铟溶液为内标制成糊状的牛肝粉。测定样品中包括挥发性元素在内的痕量元素的浓度，并与认证值相符。接下来，使用切片方法制备小鼠肝脏。包含恒定面积硫的聚苯硫醚（PPS）膜被用作定量分析的支持材料。将使用切片法制备的小鼠肝脏中的痕量元素浓度与使用普通硝酸法进行比较。仅在使用切片法获得的样品中确定了一些挥发性元素的浓度。使用切片机低温恒温器的制备方法可用于制备用于PIXE分析的生物样品。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号