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首页> 外文期刊>International Journal of Plant Breeding and Genetics >RAPD analysis for detection and fingerprinting of sunflower mutants induced by sodium azide.
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RAPD analysis for detection and fingerprinting of sunflower mutants induced by sodium azide.

机译：RAPD分析用于检测叠氮化钠诱导的向日葵突变体。

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The aim of this study was to identify and fingerprint the mutants of sunflower plants using RAPD analysis. Ten mutants of two cultivars Giza 1 and Giza 102 of Helianthus annuus produced by sodium azide treatments and the control were evaluated by RAPD markers. The mutants of cultivar Giza 102 were as follows: mutant 1 which changes in the form of inflorescence, mutant 2 is a dwarfed plant, mutant 3 is a plant with large leaves, mutant 4 is a plant with many inflorescences, mutant 5 is a dwarfed plant also. The mutants of cultivar Giza 1 were as follows: mutant 6 with reddish leaf petiole, mutant 7 is a dwarfed plant, mutant 8 with changes in the leaf form, mutant 9 is a plant with dark green inflorescence calyx, mutant 10 is a plant with stripped ray florets. Ten random primers were used; eight out of ten primers used showed variations among the mutants. The 98 amplified fragments were yielded, 83 bands were polymorphic and the total polymorphic rate was 84.6%. Some fragments were common for both genotypes and others were absent as a result of treatments. A dendrogram based on the genetic distance grouped the mutants and the two control cultivars to three clusters A, B and C. The polymorphism detected in all the mutants with both genotypes suggested that sodium azide could be used as effective mutagenic agents in sunflower. RAPD marker is a powerful technique enables fingerprinting of selected mutants in breeding of sunflower plants.
机译：这项研究的目的是使用RAPD分析来鉴定和鉴定向日葵植物的突变体。用RAPD标记评估了叠氮化钠处理产生的两个品种向日葵的Giza 1和Giza 102的十个突变体和对照。栽培品种吉萨102的突变体如下：以花序形式改变的突变体1，突变体2是矮化的植物，突变体3是有大叶子的植物，突变体4是有许多花序的植物，突变体5是矮化的植物植也。品种吉萨1的突变体如下：突变体6带有微红的叶柄，突变体7是矮化的植物，突变体8具有叶形的变化，突变体9是具有深绿色花萼的植物，突变体10是具有剥去的射线小花。使用了十个随机引物。十分之八的引物显示出突变体之间的差异。产生98个扩增片段，多态性83条带，总多态性率为84.6％。一些片段对于两种基因型都是常见的，而其他片段则由于治疗而缺失。基于遗传距离的树状图将突变体和两个对照品种分为A，B和C三个簇。在所有具有两种基因型的突变体中检测到的多态性表明，叠氮化钠可以用作向日葵的有效诱变剂。 RAPD标记是一项强大的技术，可在向日葵植物育种中对选定突变体进行指纹识别。

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