首页> 外文期刊>International Journal of Plant Breeding and Genetics >Adaptability of Litchi Germplasm in Hilly Areas of Sylhet Agricultural University and Screening their Genetic Variation by Using RAPD Markers
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Adaptability of Litchi Germplasm in Hilly Areas of Sylhet Agricultural University and Screening their Genetic Variation by Using RAPD Markers

机译:锡尔赫特农业大学丘陵地区荔枝种质的适应性及利用RAPD标记筛选其遗传变异

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Ten germplasm of litchi were collected from different eco-geographic regions of Bangladesh to evaluate their adaptation in hilly areas and screening the genetic variability both in morphological and molecular characteristics by RAPD markers. The morphological data was conducted in Randomized Complete Block Design (RCBD) with three replications and found the significant differences at all stages. Among the germplasm, the maximum number of branches per plant (14.33) and number of leaves per plant (66.33) were found in Mongalbaria which were statistically similar to BARI litchi-2 13.67 and 61.67, respectively. The germplasm, Malaysian recorded the highest plant height (68.40 cm) and the lowest trunk diameter (1.77 cm), while China-3 gave the highest trunk diameter (3.23 cm). So, by assessing morphological parameters, Mongalbaria and BARI litchi-2 have a bright prospect for growing under the hilly areas in Sylhet region. In Random Amplified Polymorphic DNA (RAPD) markers analysis, primer OPB-04 showinggood technical resolution and sufficient variation among germplasm and produced a total of 7 RAPD markers of which 6 (85.71%) were considered as polymorphic loci. The highest genetic distance (1.9459) was observed in Bombai vs. Malaysian varietals pairwhereas, the lowest genetic distance (0.1234) was estimated in BARI litch-2 vs. BARI litch-3 varietals pair. The UPGMA dendrogram showed that 10 germplasm of litchi could be classified into different groups or clusters according to their genetic similarities and dissimilarities. Two germplasm Bombai and Malaysian were found the highest genetic distance than the others that ultimately helpful for further hybridization program.
机译:从孟加拉国的不同生态地理区域收集了10个荔枝种质,以评估它们在丘陵地区的适应性,并通过RAPD标记筛选其形态和分子特征的遗传变异。形态学数据在随机完全区组设计(RCBD)中进行,重复3次,发现在所有阶段均存在显着差异。在种质中,Mongalbaria发现每株植物的最大分支数(14.33)和每株植物的叶子数(66.33),在统计学上分别类似于BARI荔枝2 13.67和61.67。马来西亚的种质最高,株高68.40 cm,树干直径最低(1.77 cm),而China-3的树干最高(3.23 cm)。因此,通过评估形态学参数,Mongalbaria和BARI荔枝2在锡尔赫特地区丘陵地区下生长具有广阔的前景。在随机扩增多态性DNA(RAPD)标记分析中,引物OPB-04显示出良好的技术分辨率和种质之间的充分变异,并产生了总共7个RAPD标记,其中6个(85.71%)被认为是多态性位点。在孟买vs.马来西亚品种对中发现了最高遗传距离(1.9459),而在BARI litch-2和BARI litch-3对中估计了最低遗传距离(0.1234)。 UPGMA树状图显示,荔枝的10个种质可以根据其遗传相似性和异同性分为不同的组或簇。发现两个种质孟买和马来西亚比其他种质具有最高的遗传距离,这最终有助于进一步的杂交程序。

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