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Molecular Mechanism of Sweet and Waxy in Maize

机译:玉米甜糯的分子机理

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摘要

The aim was to uncover the molecular mechanism of sweet and waxy in maize, hopping it to be useful in special-used maize breeding and developing some intragenic selection markers. Sweet maize and waxy maize are popular vegetable food and important materials for processing production. Their sweet and waxy characters are controlled, respectively by recessive genes sh_2, bt_1, bt_2 and wx located at different chromosome loci. On molecular level, these recessive genes are mutated from the encoding genesof the key enzymes of starch biosynthesis pathway by the insertion of different transposon elements. The effort to breed double recessive sweet-waxy maize controlled by recessive sweet genes and recessive waxy gene is impractical, because sweet and waxyphenotypes can not be expressed in the same inbred line or hybrid for their exclusion in biochemical metabolism. The backcrossing procedure of the conventional breeding method for sweet maize and waxy maize can be cut down almost to half, if intragenic selection marker is developed on the basis ' of sequence difference between the dominant genes and the recessive mutated genes and used to identify the recessive genes from the segregating generations.
机译:目的是揭示玉米中甜糯的分子机制,希望将其用于特殊用途的玉米育种并开发一些基因内选择标记。甜玉米和糯玉米是受欢迎的蔬菜食品,是加工生产的重要原料。它们的甜味和糯性分别由位于不同染色体基因座的隐性基因sh_2,bt_1,bt_2和wx控制。在分子水平上,通过插入不同的转座子元件,这些隐性基因从淀粉生物合成途径的关键酶的编码基因中突变。培育由隐性甜基因和隐性蜡质基因控制的双隐性甜蜡质玉米的努力是不切实际的,因为不能将甜型和蜡质表型排除在同一自交系或杂种中而将其排除在生化代谢之外。如果基于优势基因和隐性突变基因之间的序列差异开发出基因选择标记,并用于鉴定隐性基因,那么甜玉米和糯玉米常规育种方法的回交过程几乎可以减少一半。来自分离世代的基因。

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