首页> 外文期刊>International Journal of Plant Breeding and Genetics >Oyster Mushrooms Species Differentiation Through Molecular Markers RAPD
【24h】

Oyster Mushrooms Species Differentiation Through Molecular Markers RAPD

机译:牡蛎蘑菇通过分子标记RAPD进行物种分化

获取原文
获取原文并翻译 | 示例
           

摘要

A RAPD methodology for differentiation and characterization of the fungal edible species Pleurotus ostreatus and Pleurotus sajor-cctju was developed. Two of the most usual methods of DNA extraction were studied. The first was based in the simultaneousprecipitation of proteins and polysaccharides by the presence of SDS (sodium dodecyl sulphate) and sodium acetate and the second by the use of cetyltrimethylammonium bromide (CTAB). Both methods presented satisfactory results and the best extraction products were obtained by the one that used SDS and sodium acetate. The study with RAPDs produced consistent DNA fragments, reproducible in different gels, serving as good markers for characterization and genetic differentiation of the studied species of mushrooms. The PCR conditions for obtaining RAPD markers were optimized with good interpretation.
机译:建立了RAPD方法用于真菌可食用菌平菇和侧耳侧耳的鉴别和鉴定。研究了两种最常用的DNA提取方法。第一种是通过存在SDS(十二烷基硫酸钠)和乙酸钠同时沉淀蛋白质和多糖,第二种是通过使用十六烷基三甲基溴化铵(CTAB)。两种方法均显示出令人满意的结果,并且使用SDS和乙酸钠的提取方法获得了最佳的提取产物。使用RAPD进行的研究产生了一致的DNA片段,可在不同的凝胶中重现,可作为表征蘑菇和研究蘑菇的遗传分化的良好标记。获得良好RAPD标记的PCR条件已得到优化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号