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首页> 外文期刊>International Journal of Radiation Biology: Covering the Physical, Chemical, Biological, and Medical Effects of Ionizing and Non-ionizing Radiations >Effect of gamma radiation on phenoloxidase pathway, antioxidant defense mechanism in Helicoverpa armigera (Lepidoptera: Noctuidae) and its implication in inherited sterility towards pest suppression
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Effect of gamma radiation on phenoloxidase pathway, antioxidant defense mechanism in Helicoverpa armigera (Lepidoptera: Noctuidae) and its implication in inherited sterility towards pest suppression

机译：γ射线对棉铃虫酚氧化酶途径，抗氧化防御机制的影响及其对遗传抑制不育的影响

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Purpose: To investigate age-correlated radiosensitivity in highly radioresistant lepidopteran pest, Helicoverpa armigera, upon exposure to ionizing radiation and to examine the irradiation impact on stress-molecular responses in F1 (first-filial) progeny of irradiated (100 Gy) male moths in relation to its reproductive behavior. Materials and methods: Efficacy of sub-lethal gamma radiation was evaluated on two markedly apart ontogenic stages, neonates and adult moths. Differential growth, reproductive behavior and stress-indicating molecular responses were examined upto F1 progeny of sub-sterilized moths. Free-radical scavenging enzymes, superoxide dismutase (SOD), catalase (CAT) and Phenoloxidase cascade enzymes, pro-phenoloxidase (PPO), its activating enzyme (PPAE) were studied in irradiated and irradiated plus microbial challenge regimen (dual-stress) by Real-time RT-PCR (reverse-transcription-polymerase-chain-reaction). Results: An inverse correlation of radiosensitivity with developmental age of insect was observed. F1 sterility was higher than parent sterility. F1 progeny exhibited protraction in development and decreased survival upon irradiation. Sex ratio in F1 progeny was skewed towards males. PPO, PPAE, SOD and CAT transcripts were downregulated upon neonate irradiation resulting in enhanced vulnerability of larvae to incidental microbial challenge. These transcripts were upregulated in F1 progeny of sub-sterilized male moths (100 Gy) upon dual-stress. Conclusions: Irradiation impact on stress-indicating molecular responses in F1 progeny is correlated with its reproductive performance. These observations will permit defining regimen having pragmatic viability of 'F1 sterility technique' for pest suppression. Gamma dose of 100 Gy would ensure balance between induced sterility of males and their field competitiveness. These parameters would facilitate integration of biocontrol strategy with parabiological 'Sterile Insect Release Technique'.
机译：目的：研究高度辐射的鳞翅目害虫Helicoverpa armigera在暴露于电离辐射后与年龄相关的放射敏感性，并研究辐照对辐照的（100 Gy）雄蛾的F1（第一孝子代）后代的应力-分子响应的影响。与它的生殖行为有关。材料和方法：在两个明显分开的个体发育阶段，即新生儿和成年蛾上，评估了亚致死性伽马射线辐射的功效。研究了亚灭菌蛾的F1子代的差异生长，生殖行为和应激指示分子反应。自由基清除酶，超氧化物歧化酶（SOD），过氧化氢酶（CAT）和酚氧化酶级联酶，酚氧化酶原（PPO），其活化酶（PPAE）在辐照和辐照加微生物攻击方案（双胁迫）下进行了研究。实时RT-PCR（逆转录聚合酶链反应）。结果：观察到放射敏感性与昆虫发育年龄呈负相关。 F1不育高于父母不育。 F1后代在发育中表现出延期并且在照射时存活降低。 F1后代的性别比例偏向男性。新生儿辐射后，PPO，PPAE，SOD和CAT转录本被下调，导致幼虫对偶然微生物攻击的脆弱性增强。在双重胁迫下，这些转录本在亚灭菌雄蛾（100 Gy）的F1后代中上调。结论：辐射对F1子代的应激指示分子反应的影响与其繁殖性能有关。这些观察结果将允许定义具有实用的“ F1无菌技术”以抑制有害生物的方案。 100 Gy的伽马剂量将确保雄性不育与其田间竞争之间的平衡。这些参数将促进生物防治策略与副生物“无菌昆虫释放技术”的整合。

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