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首页> 外文期刊>International Journal of Radiation Biology: Covering the Physical, Chemical, Biological, and Medical Effects of Ionizing and Non-ionizing Radiations >Effect of expression of the Escherichia coli nth gene in Saccharomyces cerevisiae on the toxicity of ionizing radiation and hydrogen peroxide.
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Effect of expression of the Escherichia coli nth gene in Saccharomyces cerevisiae on the toxicity of ionizing radiation and hydrogen peroxide.

机译：酿酒酵母中大肠杆菌nth基因表达对电离辐射和过氧化氢毒性的影响。

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摘要

PURPOSE: To examine the contribution of endonuclease III (Nth)-repairable lesions to the cytotoxicity of ionizing radiation (IR) and hydrogen peroxide (H2O2) in the yeast Saccharomyces cerevisiae. MATERIALS AND METHODS: A selectable expression vector containing the E. coli nth gene was transformed into two different wild-type strains (7799-4B and YNN-27) as well as one rad52 mutant strain (C5-6). Nth expression was verified by Western analysis. Colony-forming assay was used to determine the sensitivity to IR and H2O2 in both stationary and exponentially growing cells. RESULTS: The pADHnth-transformed wild-type (77994B) strain was considerably more resistant than vector-only transformants to the toxic effects of IR, in both stationary and exponential growth phases, although this was not the case in another wild-type strain (YNN-27). In contrast, there were no significant effects of nth expression on the sensitivity of the wild-type cells to H2O2. Moreover, nth expression caused no effects on the H2O2 sensitivity in the rad52 mutant cells, but it led to a slight increase in sensitivity in these cells following IR, particularly at the highest dose levels used. CONCLUSIONS: Whilst other damage-processing systems may play a role, DNA lesions that are substrates for Nth can also make a contribution to the toxic effects of IR in certain wild-type yeast. Hence, DNA double-strand breaks should not be considered the sole lethal lesions following IR exposure.
机译：目的：研究内切酶III（Nth）可修复性损伤对啤酒酵母中电离辐射（IR）和过氧化氢（H2O2）的细胞毒性的影响。材料与方法：将含有大肠杆菌nth基因的选择性表达载体转化为两种不同的野生型菌株（7799-4B和YNN-27）以及一种rad52突变菌株（C5-6）。通过Western分析验证了第N个表达。集落形成测定法用于测定静止和指数生长细胞对IR和H2O2的敏感性。结果：在静止和指数生长期，pADHnth转化的野生型（77994B）菌株对IR的毒性作用均比纯载体转化株高得多，尽管在另一种野生型菌株中则没有（ YNN-27）。相反，nth表达对野生型细胞对H2O2的敏感性没有显着影响。此外，第n个表达对rad52突变细胞中H2O2的敏感性没有影响，但是导致IR后这些细胞中的敏感性略有增加，特别是在使用最高剂量水平时。结论：虽然其他损伤处理系统可能也起作用，但作为Nth底物的DNA损伤也可能对某些野生型酵母中IR的毒性产生影响。因此，不应将DNA双链断裂视为IR暴露后唯一的致死性病变。

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