• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Radiation Biology: Covering the Physical, Chemical, Biological, and Medical Effects of Ionizing and Non-ionizing Radiations >Rejoining of double-stranded DNA-fragments studied in different size-intervals.
【24h】

Rejoining of double-stranded DNA-fragments studied in different size-intervals.

机译：在不同的大小间隔研究双链DNA片段的重新加入。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

PURPOSE: To measure rejoining of radiation-induced doublestranded DNA-fragments of different sizes and to evaluate the effects of size-resolution in the analysis of rejoining. MATERIAL AND METHODS: Normal human fibroblasts (GM5758) were irradiated with photons or accelerated nitrogen ions (linear energy transfer, LET = 125 keV microm(-1)) and incubated for repair for 0-22 h. Double-stranded DNA-fragments from the irradiated cells were separated by pulsed-field gel electrophoresis in the range approximately 5 kbp to 5.7 Mbp. RESULTS: For cells irradiated with high LET nitrogen ions, there was an increase in the fast half-time from approximately 5 min for fragments < 400 kbp to 10 min when all fragments < 5.7 Mbp were measured. Further, the fraction of fragments rejoined by the slow-rejoining phase increased significantly for increased threshold sizes. The fraction of unrejoined fragments after 22 h and the half-time for the slow-rejoining phase remained constant for all threshold sizes. For cells irradiated with lower doses of low LET radiation the rejoining was shifted towards a slower kinetics when fragments up to 10 Mbp were excluded in the analysis. CONCLUSION: DNA exclusion-size and resolution may affect the estimates of DNA double-strand break rejoining. Using a low-resolution technique that does not detect small fragments will result in an underestimation, or even disappearance of the fast-rejoining phase. This is due to substantial rejoining of fragments taking place before the fragments are of sufficient size to be monitored.
机译：目的：测量不同尺寸的辐射诱导的双链DNA片段的重新结合，并评估尺寸分辨率在重新结合分析中的作用。材料与方法：用光子或加速的氮离子（线性能量转移，LET = 125 keV microm（-1））照射正常人成纤维细胞（GM5758），并保温0-22小时。来自被照射细胞的双链DNA片段通过脉冲场凝胶电泳在大约5kbp至5.7Mbp的范围内分离。结果：对于用高LET氮离子辐照的细胞，快速半衰期从小于400 kbp的片段的大约5分钟增加到测量所有小于5.7 Mbp的片段的10分钟。此外，随着阈值大小的增加，由慢速重新结合阶段重新结合的片段比例也显着增加。对于所有阈值大小，慢速重新结合阶段的22小时和半时间后未重新结合的碎片比例均保持不变。对于用较低剂量的低LET辐射辐照的细胞，当分析中排除高达10 Mbp的片段时，重新结合朝着较慢的动力学转变。结论：DNA排阻的大小和分辨率可能影响DNA双链断裂重新结合的估计。使用无法检测小碎片的低分辨率技术会导致低估，甚至导致快速重新加入阶段的消失。这是由于在碎片具有足够的大小以进行监视之前，发生了碎片的大量重新结合。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号