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首页> 外文期刊>British Poultry Science >Molecular cloning, expression and polymorphism of goose LRP8 gene
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Molecular cloning, expression and polymorphism of goose LRP8 gene

机译：鹅LRP8基因的分子克隆，表达及多态性

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1. The low-density lipoprotein receptor-related protein 8 (LRP8), a member of the low-density lipoprotein receptor (LDLR) gene family, participates in the supplying of lipid during follicular development. The objective of the study was to identify and characterise the LRP8 gene in goose.2. A 2867 bp fragment that covered the complete coding region (CDS) of goose (Anser cygnoides) LRP8 gene was cloned. It encoded a protein of 917 amino acid residues containing a 24-amino acid signal peptide and 5 functional domains. The goose LRP8 showed high nucleic acid and amino acid identities with those in other species.3. Similarly to duck LRP8 gene, two splice variants of LRP8, LRP8-1 (containing 8 ligand-binding repeats) and LRP8-2 (containing 7 ligand-binding repeats), were identified in goose.4. Semi-quantitative RT-PCR analysis indicates that the LRP8-1 transcript is expressed in heart, liver, spleen, lung, kidney, breast muscle, duodenum, hypothalamus, pituitary and ovary, negligible or absent in sebum and oviduct, and the LRP8-2 transcript is widely expressed in all examined tissues.5. A total of 7 SNPs were identified in the coding region of the goose LRP8 gene
机译：1.低密度脂蛋白受体相关蛋白8（LRP8）是低密度脂蛋白受体（LDLR）基因家族的成员，在卵泡发育过程中参与脂质的供应。本研究的目的是鉴定和表征鹅中的LRP8基因。2。克隆了一个覆盖鹅（Anser cygnoides）LRP8基因完整编码区（CDS）的2867 bp片段。它编码了一个917个氨基酸残基的蛋白质，其中包含一个24个氨基酸的信号肽和5个功能域。鹅LRP8与其他物种的核酸和氨基酸具有高度同一性。3。与鸭LRP8基因相似，在鹅中鉴定出LRP8的两个剪接变体LRP8-1（包含8个配体结合重复序列）和LRP8-2（包含7个配体结合重复序列）4。半定量RT-PCR分析表明LRP8-1转录本在心脏，肝脏，脾脏，肺，肾，胸肌，十二指肠，下丘脑，垂体和卵巢中表达，皮脂和输卵管微不足道或缺失，LRP8-在所有检查过的组织中均广泛表达2个转录本。5。在鹅LRP8基因的编码区共鉴定出7个SNP

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