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首页> 外文期刊>International journal of sports medicine >Effects of high intensity exercise on isoelectric profiles and SDS-PAGE mobility of erythropoietin.
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Effects of high intensity exercise on isoelectric profiles and SDS-PAGE mobility of erythropoietin.

机译：高强度运动对促红细胞生成素等电图和SDS-PAGE迁移率的影响。

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摘要

Exercise induced proteinuria is a common phenomenon in high performance sports. Based on the appearance of so called "effort urines" in routine doping analysis the purpose of this study was to investigate the influence of exercise induced proteinuria on IEF profiles and SDS-PAGE relative mobility values (rMVs) of endogenous human erythropoietin (EPO). Twenty healthy subjects performed cycle-ergometer exercise until exhaustion. VO (2)max, blood lactate, urinary proteins and urinary creatinine were analysed to evaluate the exercise performance and proteinuria. IEF and SDS-PAGE analyses were performed to test for differences in electrophoretic behaviour of the endogenous EPO before and after exercise. All subjects showed increased levels of protein/creatinine ratio after performance (8.8+/-5.2-26.1+/-14.4). IEF analysis demonstrated an elevation of the relative amount of basic band areas (13.9+/-11.3-36.4+/-12.6). Using SDS-PAGE analysis we observed a decrease in rMVs after exercise and no shift in direction of the recombinant human EPO (rhEPO) region (0.543+/-0.013-0.535+/-0.012). Following identification criteria of the World Anti Doping Agency (WADA) all samples were negative. The implementation of the SDS-PAGE method represents a good solution to distinguish between results influenced by so called effort urines and results of rhEPO abuse. Thus this method can be used to confirm adverse analytical findings.
机译：运动诱发的蛋白尿是高性能运动中的常见现象。基于常规兴奋剂分析中所谓“努力尿液”的出现，本研究的目的是研究运动诱发的蛋白尿对内源性人类促红细胞生成素（EPO）的IEF谱和SDS-PAGE相对迁移率值（rMVs）的影响。二十名健康受试者进行了骑自行车测力计运动，直至疲惫。对VO（2）max，血液乳酸，尿蛋白和尿肌酐进行分析，以评估运动表现和蛋白尿。进行了IEF和SDS-PAGE分析，以测试运动前后内源性EPO的电泳行为差异。所有受试者表现出蛋白质/肌酐比值的水平增加（8.8 +/- 5.2-26.1 +/- 14.4）。 IEF分析表明，基带区域的相对数量增加了（13.9 +/- 11.3-36.4 +/- 12.6）。使用SDS-PAGE分析，我们观察到运动后rMV降低，并且重组人EPO（rhEPO）区域的方向没有变化（0.543 +/- 0.013-0.535 +/- 0.012）。根据世界反兴奋剂机构（WADA）的鉴定标准，所有样品均为阴性。 SDS-PAGE方法的实施代表了一种区分受费力尿影响的结果和rhEPO滥用结果的好方法。因此，该方法可用于确认不利的分析结果。

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