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首页> 外文期刊>Applied Magnetic Resonance >Investigation of metabolic changes in blood and tissue of mice gamma-irradiated with sublethal doses by direct observation of EPR signals from Hb-NO complexes
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Investigation of metabolic changes in blood and tissue of mice gamma-irradiated with sublethal doses by direct observation of EPR signals from Hb-NO complexes

机译：通过直接观察Hb-NO配合物的EPR信号研究用亚致死剂量γ辐照的小鼠血液和组织的代谢变化

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The metabolic changes in probes of blood and tissue (spleen, liver and kidney) of mice under total gamma-irradiation with the doses varied in the interval of 1-10 Gy at the dose rate of 0.073 Gy/min were studied in the early postirradiation period by ex vivo electron paramagnetic resonance (EPR). It was established that the impact with the lower dose rate leads to more intensive nitric monoxide biosynthesis in comparison with higher dose rates. In the early postirradiation period (from 2 up to 6 h), irradiation with doses higher than 2 Gy brings about an increase of the NO concentration and, hence, the appearance of nitrosyl complexes which were registered directly by EPR in blood and spleen. The observed line is identified as the signal from alpha-(Fe2+-NO2 beta(Fe3+)(2) or alpha-(Fe2+-NO)alpha(Fe2+)beta(Fe3+)(2) complexes since the methemoglobin concentration also increases in comparison with the control level. The concentration of Hb-NO complexes in blood and spleen depends on the dose and individual radiosensitivity of the organism. Therefore; the intensity of the Hb-NO signal may serve as a criterion of the radiation injury level during the first hours after the irradiation. 30 h after the impact, the Hb-NO complexes were no longer detected. For the first day, the concentration of Fe3+-transferrin in blood increases with the dose and time passed after the irradiation. The intensity of the EPR signal from Fe3+-transferrin in blood may also serve as a measure of the radiation injury level.
机译：在辐照后早期研究了在总伽马射线辐照下，小鼠的血液和组织（脾，肝和肾）探针的代谢变化，剂量在1-10 Gy范围内变化，剂量率为0.073 Gy / min。体外电子顺磁共振（EPR）。已经确定，与较高剂量率相比，较低剂量率的影响导致更强烈的一氧化氮生物合成。在辐照后的早期阶段（从2到6 h），辐照剂量大于2 Gy会导致NO浓度增加，因此亚硝酰复合物的出现会被EPR直接记录在血液和脾脏中。观察到的谱线被识别为来自alpha-（Fe2 + -NO2 beta（Fe3 +）（2）或alpha-（Fe2 + -NO）alpha（Fe2 +）beta（Fe3 +）（2）配合物的信号，因为相比之下，高铁血红蛋白浓度也有所增加血液和脾脏中Hb-NO络合物的浓度取决于生物体的剂量和个体放射敏感性，因此，Hb-NO信号的强度可作为第一阶段放射损伤水平的标准照射后30小时，撞击后30小时，不再检测到Hb-NO络合物;第一天，血液中Fe3 +-转铁蛋白的浓度随剂量和照射时间的延长而增加。血液中Fe3 +-转铁蛋白的信号也可以作为辐射损伤水平的量度。

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