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Water transbilayer diffusion in macroscopically oriented lipid bilayers as studied by pulsed field gradient NMR

机译:通过脉冲场梯度核磁共振研究宏观取向的脂质双层中水的双分子层扩散

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摘要

Water self-diffusion in lipid bilayers macroscopically oriented on glass plates was studied by pulsed field gradient H-1 nuclear magnetic resonance technique. Diffusion decays were multicomponent with a distribution of diffusion coefficients ranging from about 10(-10) to about 10(-13) m(2)/s. A number of measurements with variations of the sample orientation, diffusion time and the distance between the glass plates showed that the '' fast '' component of diffusion corresponds to water in the bilayer '' cracks ''. The '' slow '' component of diffusion corresponds to transbilayer water diffusion in the long-diffusion-time regime. For a more reliable separation of parts corresponding to fast and slow diffusion of water, a '' component-resolved spectroscopy '' method for the global analysis of correlated spectral data (P. Stilbs, K. Paulsen, P.C. Griffiths: J. Phys. Chem. 100, 8180, 1996) was applied.
机译:利用脉冲场梯度H-1核磁共振技术研究了在玻璃板上宏观取向的脂质双层中水的自扩散。扩散衰减是多组分的,扩散系数的分布范围从大约10(-10)到大约10(-13)m(2)/ s。随样品取向,扩散时间和玻璃板之间距离的变化而进行的许多测量表明,扩散的“快速”成分对应于双层“裂缝”中的水。扩散的“慢”成分对应于长时间扩散过程中的跨双层水扩散。为了更可靠地分离与水的快速扩散和缓慢扩散相对应的零件,可以使用``组分分辨光谱''方法对相关光谱数据进行全局分析(P.Stilbs,K.Paulsen,PC Griffiths:J.Phys。 Chem.100,8180,1996)。

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