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首页> 外文期刊>Medycyna Weterynaryjna >Applying the isothermal DNA amplification method in identifying the Marek's disease virus in poultry
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Applying the isothermal DNA amplification method in identifying the Marek's disease virus in poultry

机译：应用等温DNA扩增法鉴定家禽马立克氏病病毒

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摘要

Marek's disease is a viral disease, a type of poultry cancer. It is caused by MDV serotype 1 (1VIarek's disease virus, MDV) viruses, also referred to as Gallid herpesvirus 2 belonging to the family Herpesviridae. The complete, fully infectious virus particles are present in the feathers and the nodules months can survive in the environment for many months. The use of isothermal amplification methods of genetic material (Loop Mediated Isothermal Amplification, LAMP) virus MDV enables rapid and precise identification of the virus in difficult diagnostic material, including dust. By optimizing the reaction conditions and using at least two pairs of primers which bind to specific sites in the genome of the virus, this technique has high sensitivity and specificity. The technique makes it possible to detect of single copies of the meq oncogene - a unique region of DNA of the first type of virus MDV.
机译：马立克氏病是一种病毒性疾病，是一种家禽癌症。它是由 MDV 血清型 1（1VIarek 病病毒，MDV）病毒引起的，也称为属于疱疹病毒科的 Gallid 疱疹病毒 2。完整的、完全传染性的病毒颗粒存在于羽毛中，结节月可以在环境中存活数月。使用遗传物质的等温扩增方法（环介导的等温扩增，LAMP）病毒MDV可以快速准确地鉴定困难诊断材料（包括灰尘）中的病毒。通过优化反应条件并使用至少两对与病毒基因组中特定位点结合的引物，该技术具有高灵敏度和特异性。该技术可以检测meq癌基因的单个拷贝 - 第一种病毒MDV的独特DNA区域。

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