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首页> 外文期刊>International medical journal: IMJ >Effect of Toilet Detergent on Morphological Change of Human Spermatozoa
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Effect of Toilet Detergent on Morphological Change of Human Spermatozoa

机译：厕所清洁剂对人精子形态变化的影响

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Objective: We encountered a case of a girl killed at her own home and found approximately ten days after death. Five persons were suspected as killer. A condom containing human semen and filled with toilet detergent was found in the toilet chamber pot. The problem was to determine the time when the semen was discarded. Therefore we needed to provide evidence to demonstrate how long the semen had been in the toilet. Design: Validity study was performed in effect of toilet detergent on morphological change of human spermatozoa. Materials and methods: Human semen samples were collected from fifteen healthy volunteers, and the samples were confirmed to be normal by routine semen examination. The semen samples were mixed in two concentrations of a common toilet detergent (0.2 mg/ml and 0.02 mg/ml), or in water as control. All these preparations were kept at room temperature and were examined periodically under a microscope. The Oppitz's method was used for spermatozoa staining. Results: The major change of spermatozoa was morphological change. Especially, separation of the tail and the head was clearly observed on day 10 under 400 X magnifications. In samples mixed with 0.2 mg/ml solution of toilet detergent, separation of the tail and head was observed in approximately 40% of the spermatozoa on day 10, 80% on day 20 and 98% on day 30. When mixed with 0.02 mg/ml of toilet detergent, the corresponding proportions were approximately 40%, 70% and 95%, respectively. In the water control, only 25% showed separation on day 10, and the percentage on days 20 and 30 was similar to 0.02 mg/ml detergent. Conclusion: The proportion of intact spermatozoa decreased with time when semen specimens were mixed with diluted toilet detergent and kept at room temperature.
机译：目标：我们遇到了一个女孩在自己家中被杀的案例，死后大约十天才发现。五人被怀疑是杀手。在马桶便池中发现一个装有人精液并装有马桶清洁剂的避孕套。问题在于确定丢弃精液的时间。因此，我们需要提供证据来证明精液已经在厕所中停留了多长时间。设计：进行了有效性研究，研究了洗洁精对人类精子形态变化的影响。材料和方法：从15名健康志愿者那里收集人精液样品，并通过常规精液检查确认样品正常。将精液样品以两种浓度的普通厕所清洁剂（0.2 mg / ml和0.02 mg / ml）混合，或在水中作为对照。所有这些制剂均保持在室温下，并在显微镜下定期检查。用Oppitz法对精子染色。结果：精子的主要变化是形态变化。特别地，在第10天在400X放大倍率下清楚地观察到尾巴和头部的分离。在混合有0.2 mg / ml洗洁精溶液的样品中，第10天观察到大约40％的精子尾巴和头部分离，第20天观察到80％的精子和第30天观察到98％的精子。毫升洗手液，相应的比例分别为约40％，70％和95％。在水质控制中，在第10天只有25％出现分离，而在第20天和第30天的百分比类似于0.02 mg / ml洗涤剂。结论：将精液标本与稀释的洗洁精混合并保持在室温下，完整精子的比例随时间降低。

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