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首页> 外文期刊>International medical journal: IMJ >Isolation of acanthamoeba spp. from contact lens paraphernalia
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Isolation of acanthamoeba spp. from contact lens paraphernalia

机译：棘阿米巴属物种的分离。来自隐形眼镜的用具

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Introduction: Acanthamoeba spp. are ubiquitous free-living protozoa that are widely distributed in all types of environment throughout the world. Acanthamoeba sp. is the causative agent of two diseases; keratitis and granulomatous amoebic encephalitis. Contaminated contact lenses and corneal lesions are the major risk factors in causing Acanthamoeba keratitis. Objective: The main objective of this study is to isolate Acanthamoeba sp. from contact lens paraphernalia. Materials and Methods: One hundred and seventy five swabs from contact lens paraphernalia were obtained, consisting of 66 swabs of contact lenses, 52 swabs of contact lens storage cases and 57 samples of contact lens disinfecting solutions. Filtration and culture techniques were used to isolate the organism using standard methods. Results: This study successfully isolated Acanthamoeba sp. from contact lenses and contact lens storage cases at 10.6% and 13.5% respectively. However no Acanthamoeba sp. was isolated from the contact lens disinfecting solution. Many of those contact lens wearers whose contact lens paraphernalia were positive for Acanthamoeba showed a deviation from contact lens wear and care procedures recommended by the lens manufacturer and health professionals. Conclusion: The findings that contact lens paraphernalia harbours Acanthamoeba is particularly worrying as the number of cases is increasing. Contact lens wearers should be educated regarding proper hygienic care of their contact lenses. ? 2013 Japan International Cultural Exchange Foundation & Japan Health Sciences University.
机译：简介：棘形棘属。是无处不在的原生动物，广泛分布于世界各地的所有类型的环境中。棘阿米巴藻是两种疾病的病因;角膜炎和肉芽肿性阿米巴性脑炎。接触镜污染和角膜病变是引起棘阿米巴角膜炎的主要危险因素。目的：本研究的主要目的是分离棘阿米巴菌。来自隐形眼镜的用具。材料和方法：从隐形眼镜用具中获得了175个拭子，包括66个隐形眼镜拭子，52个隐形眼镜存储盒拭子和57个隐形眼镜消毒液样品。过滤和培养技术用于使用标准方法分离生物。结果：本研究成功分离出棘阿米巴菌。分别来自隐形眼镜和隐形眼镜收纳盒的10.6％和13.5％。但是没有棘阿米巴菌属。从隐形眼镜消毒溶液中分离出来。那些隐形眼镜用具中的棘阿米巴阳性的隐形眼镜佩戴者中，许多人都偏离了隐形眼镜制造商和健康专业人员建议的隐形眼镜佩戴和护理程序。结论：随着病例数的增加，隐形眼镜用具中带有棘阿米巴的发现尤其令人担忧。应教育隐形眼镜配戴者有关隐形眼镜的适当卫生护理的知识。 ？ 2013年日本国际文化交流基金会和日本卫生科学大学。

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