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首页> 外文期刊>British journal of neurosurgery >Towards a glioma model for surgical technique evaluation in the rat
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Towards a glioma model for surgical technique evaluation in the rat

机译：建立用于神经胶质瘤模型的大鼠手术技术评估

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Introduction. Evaluation of new surgical techniques in animal models is frequently challenging. This article describes the pitfalls, peculiarities and the final best applicable model for evaluating surgical techniques for glioma resection. Methods. The C6 glioma cell line and the Sprague-Dawley rat strain were selected. Fifty-thousand glioma cells were stereotactically transplanted in the left hemisphere of 137 male adult rats. Evaluation of solid tumour formation, tumour growth and scheduling of surgical resection was performed by MR scanning at 1, 2, and 4 weeks after transplantation and 3 and 6 months after tumour resection. Microsurgical tumour resection was performed with conventional techniques or with the waterjet dissector at a pressure of 6 bar. One subgroup of each surgical technique was sacrificed directly after surgery for histological analysis. The other subgroup was followed up for long-term analysis. Results. The transplantation site was of great importance. After transplantation of tumour cells posterior to the bregma, intra-ventricular tumour growth with spreading occurred. Homogenous and reproducible tumour growth was achieved after grafting cells lateral - 3 mm, anterior + 1 mm, and - 2.5 mm ventral to the bregma. After development of solid tumours on MR imaging, animals were subjected to surgery. MR and intra-operative findings corresponded well. However, MRI and intra-operative none-detectable perivascular tumour spreading was histologically observed in the majority of cases. Conclusions. The presented glioma rat model consisting of the C6 cell line and Sprague-Dawley rats as recipients is a well-suited model to investigate surgical techniques and their impact on tumour therapy. However, the site of transplantation, the preparation of cell grafts and the technique of tumour growth evaluation is of utmost importance to achieve reliable results.
机译：介绍。在动物模型中评估新的外科手术技术通常具有挑战性。本文介绍了评估神经胶质瘤切除手术技术的陷阱，特点和最终最佳适用模型。方法。选择C6神经胶质瘤细胞系和Sprague-Dawley大鼠品系。在137只雄性成年大鼠的左半球中立体定向移植了5万个神经胶质瘤细胞。在移植后1、2和4周以及肿瘤切除后3和6个月通过MR扫描评估实体瘤的形成，肿瘤生长和手术切除的时间表。用常规技术或在6 bar的压力下用水刀解剖仪进行显微外科肿瘤切除。每种手术技术的一个亚组在手术后直接处死以进行组织学分析。另一个亚组进行了长期分析。结果。移植部位非常重要。在前reg后的肿瘤细胞移植后，发生脑室内肿瘤扩散。将细胞横向移植至前b腹侧3 mm，前+1 mm和-2.5 mm后，可实现均质且可复制的肿瘤生长。在MR成像上发展为实体瘤之后，对动物进行手术。 MR和术中发现非常吻合。然而，在大多数情况下，组织学观察到MRI和术中无法检测到的血管周围肿瘤扩散。结论提出的由C6细胞系和Sprague-Dawley大鼠作为受者组成的神经胶质瘤大鼠模型是研究手术技术及其对肿瘤治疗影响的合适模型。然而，移植部位，细胞移植物的制备以及肿瘤生长评估技术对于获得可靠的结果至关重要。

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