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A Phylogenetic Index for Cichlid Microsatellite Primers

机译:丽鱼科鱼微卫星引物的系统发育指数。

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Microsatellites abound in most organisms and have proven useful for a range of genetic and genomic studies. Once primers have been created, they can be applied to populations or taxa that have diverged from the source taxon. We use PCR amplification,in a 96-well format, to determine the presence and absence of 46 microsatellite loci in 13 cichlid species. At least one primer set amplified a product in each species tested, and some products were present in nearly all species. These results are compared to the known phylogenetic relationships among cichlids. While we do not address intraspecies variation, our results present a phylogenetic index for the success of microsatellite PCR primer product amplification, thus providing information regarding a collection of primers that are applicable to wide range of species. Through the use of such a uniform primer panel, the potential impact for cross species would be increased.
机译:微卫星在大多数生物中比比皆是,并且已被证明可用于一系列遗传和基因组研究。一旦创建了引物,就可以将其应用于与源分类单元不同的种群或分类单元。我们以96孔格式使用PCR扩增,确定13种丽鱼科动物中是否存在46个微卫星基因座。至少一个引物组在每个测试物种中扩增了一种产物,并且几乎所有物种中都存在一些产物。将这些结果与丽鱼科鱼之间已知的系统发育关系进行了比较。虽然我们没有解决物种内变异问题,但我们的结果为微卫星PCR引物产物扩增的成功提供了系统发育指标,从而提供了适用于多种物种的引物信息。通过使用这种均匀的底漆面板,将增加对交叉物种的潜在影响。

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