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首页> 外文期刊>International Journal of Zoology >Expression Pattern of Myogenic Regulatory Transcription Factor mRNAs in the Embryo and Adult Labeo rohita (Hamilton, 1822)
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Expression Pattern of Myogenic Regulatory Transcription Factor mRNAs in the Embryo and Adult Labeo rohita (Hamilton, 1822)

机译：胚胎和成年Labeo rohita中肌源性调控转录因子mRNA的表达模式（Hamilton，1822）

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摘要

Understanding the regulation of skeletal muscle development is important to meet the increasing demand of Indian major carp Labeo rohita. Myogenic regulatory factors (MRFs) along with myocyte specific enhancer factor 2 (MEF2) play the pivotal role inthe determination and differentiation of skeletal muscle. The majority of skeletal muscle genes require both MRFs and MEF2 family members to activate their transcription. In this study, the expression pattern of MyoD, myf-5, myogenin, and MEF2A was observed from 6 h after fertilization to 12 months of age using semiquantitative RT-PCR as well as real-time PCR method. MyoD and myf-5 mRNAs were expressed at high level at the early embryonic stages. Myogenin and MEF2A were expressed after MyoD and myf-5 and remained active up to adult stage. Expression of MyoD was lower than that of Myf-5 after the 5th month. Partial sequencing of MyoD, myf-5, and MEF2A was done to draw phylogeny. In phylogenetic study, Labeo MyoD, MEF2A and myf-5 were found to be closely related to those of common carp. The present investigation suggests that the four transcription factors play pivotal role in the regulation of muscle growth of Labeo rohita in an overlapping and interconnected way.
机译：了解骨骼肌发育的规律对于满足印度主要鲤鱼Labeo rohita的不断增长的需求非常重要。肌源性调节因子（MRF）以及肌细胞特异性增强因子2（MEF2）在骨骼肌的确定和分化中起关键作用。大多数骨骼肌基因都需要MRF和MEF2家族成员来激活其转录。在这项研究中，使用半定量RT-PCR和实时PCR方法，从受精后6小时到12个月大时观察到了MyoD，myf-5，肌生成素和MEF2A的表达模式。 MyoD和myf-5 mRNA在胚胎早期阶段高水平表达。 MyoD和myf-5之后，Myogenin和MEF2A表达，直到成年阶段仍保持活跃。第5个月后，MyoD的表达低于Myf-5。对MyoD，myf-5和MEF2A进行了部分测序以绘制系统发育图。在系统发育研究中，发现Labeo MyoD，MEF2A和myf-5与鲤鱼密切相关。目前的研究表明，这四个转录因子在重叠和相互联系的方式在Labeo rohita肌肉生长的调节中起关键作用。

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