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首页> 外文期刊>Inorganica Chimica Acta >Spectroscopic study of the compound ES and the oxoferryl compound II states of cytochrome c peroxidase: comparison with the compound II of horseradish peroxidase
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Spectroscopic study of the compound ES and the oxoferryl compound II states of cytochrome c peroxidase: comparison with the compound II of horseradish peroxidase

机译：化合物ES和草酰氧化合物II的细胞色素C过氧化物酶状态的光谱研究：与辣根过氧化物酶的化合物II比较

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摘要

Magnetic circular dichroism (MCD) spectroscopic data at -30 degrees C are reported for the oxoferryl compound II state of cytochrome c peroxidase (CcP), which was generated by hydrogen peroxide oxidation of ferrous CcP, in comparison with compound ES (an oxoferryl heme with a protein based-radical) of CcP and compound II (an oxoferryl heme) of horseradish peroxidase (HRP-II). Detailed spectral analyses reveal that the protein-based free radical found in CcP-ES does not significantly perturb the electronic environment of the heme as measured by electronic absorption and MCD spectroscopy. Thus, the spectra of the compounds I and II (oxoferryl) states of CcP are similar. The compound I state of HRP (HRP-I), on the other hand, contains an oxoferryl heme coupled to a porphyrin pi-cation radical. As a consequence, HRP-I and HRP-II are well known to have quite distinctive spectra. The similarity of the spectral properties of the two active species of CcP, and the contrasting dissimilarity between the spectral properties of the two active high valent species of HRP, clearly indicates the importance of the protein structure surrounding the heme group in determining the spectral properties of the active species. (C) 1998 Elsevier Science S.A. All rights reserved. [References: 37]
机译：报告了在30摄氏度下的圆环二色性（MCD）的电磁波谱数据，与化合物ES（过氧化血红素血红素与CcP和辣根过氧化物酶（HRP-II）的化合物II（草酰血红素）的蛋白质基自由基。详细的光谱分析表明，在CcP-ES中发现的基于蛋白质的自由基不会显着干扰血红素的电子环境，如通过电子吸收和MCD光谱法所测。因此，CcP的化合物I和II（氧氟苯乙烯）态的光谱相似。另一方面，HRP的化合物I状态（HRP-1）包含与卟啉π-阳离子自由基偶联的草酰氧血红素。结果，众所周知，HRP-I和HRP-II具有非常独特的光谱。 CcP的两种活性物质的光谱性质相似，以及HRP的两种活性高价物质的光谱性质之间的对比差异，清楚地表明，血红素基团周围的蛋白质结构在确定Hp的光谱性质方面的重要性。活跃的物种。 （C）1998 Elsevier Science S.A.保留所有权利。 [参考：37]

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