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首页> 外文期刊>Investigative radiology >Gadolinium-fullerenol as a paramagnetic contrast agent for cellular imaging.
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Gadolinium-fullerenol as a paramagnetic contrast agent for cellular imaging.

机译：full富勒烯醇作为顺磁性造影剂用于细胞成像。

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OBJECTIVES: The objectives of this study were to test cell-labeling methods to achieve intracellular labeling and T1 enhancement of cells on magnetic resonance imaging using a paramagnetic Gd@C82 fullerenol contrast agent, and to determine the effect of labeling on cell viability, metabolism, and differentiation capacity. MATERIALS AND METHODS: We tested the use of a transfection agent for labeling cells in culture with Gd@C82 fullerenol. Proliferation, viability, and differentiation assays of mesenchymal stem cell (MSC) cultures; light and electron microscopy of MSC and macrophages; and MRI of MSC, macrophage, and HeLa cervical carcinoma cell cultures in vitro and in vivo were performed to evaluate the labeled cells. RESULTS: Protamine sulfate transfection increased cell uptake of Gd@C82 fullerenols. The label was distributed in endosomes in the cytoplasm as shown by electron microscopy. High viability was shown for all cell lines and normal differentiation capacity was shown for MSCs. T1 of labeled MSC at 7 T was reduced 71% compared with unlabeled cells. CONCLUSIONS: Cellular labeling with Gd@C82 is feasible and can produce T1-enhanced cells on magnetic resonance imaging. This study suggests that further investigation of Gd fullerenols for tracking studies of viable cells, including stem cells, is warranted.
机译：目的：本研究的目的是测试细胞标记方法，以使用顺磁性Gd @ C82富勒烯醇造影剂在磁共振成像中实现细胞内标记和T1增强，并确定标记对细胞活力，代谢，和分化能力。材料与方法：我们测试了用Gd @ C82富勒烯醇标记培养物中细胞的转染剂的使用。间充质干细胞（MSC）培养物的增殖，活力和分化测定; MSC和巨噬细胞的光学和电子显微镜观察;进行了体外和体内MSC，巨噬细胞和HeLa宫颈癌细胞培养的MRI和MRI，以评估标记的细胞。结果：硫酸鱼精蛋白转染增加了Gd @ C82富勒烯醇的细胞摄取。如电子显微镜所示，标记物分布在细胞质的内体中。在所有细胞系中均显示出高存活力，并且对于MSC显示出正常的分化能力。与未标记的细胞相比，标记的MSC在7 T时的T1降低了71％。结论：用Gd @ C82进行细胞标记是可行的，并且可以在磁共振成像中产生T1增强的细胞。这项研究表明，有必要对Gd富勒烯醇进行进一步研究以追踪对包括干细胞在内的活细胞的研究。

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