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首页> 外文期刊>Bioelectromagnetics. >Millimeter wave exposure reverses TPA suppression of gap junction intercellular communication in HaCaT human keratinocytes.
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Millimeter wave exposure reverses TPA suppression of gap junction intercellular communication in HaCaT human keratinocytes.

机译:毫米波暴露逆转了TCA对HaCaT人角质形成细胞中间隙连接细胞间通讯的抑制作用。

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摘要

The effect of 30.16 GHz millimeter wave (MMW) exposure at 1.0 and 3.5 mW/cm(2) on gap junction intercellular communication (GJIC) was studied in cultured HaCaT keratinocytes, using the fluorescence recovery after photobleaching (FRAP) technique and laser confocal scanning microscopy to follow the intracellular movement of 5,6-carboxyfluorescein diacetate dye. While MMW exposure alone for 1 h at either 1.0 or 3.5 mW/cm(2) did not affect GJIC, MMW exposure in combination with 5 ng/ml TPA treatment reversed TPA induced suppression of GJIC. Exposure at 1.0 mW/cm(2) resulted in a partial reversal, and exposure at 3.5 mW/cm(2) resulted in essentially full reversal of the TPA suppression. Bioelectromagnetics 25:1-4, 2004.
机译:使用光漂白后的荧光恢复(FRAP)技术和激光共聚焦扫描技术,研究了培养的HaCaT角质形成细胞中间隙连接细胞间通讯(GJIC)在1.0和3.5 mW / cm(2)下暴露30.16 GHz毫米波(MMW)的影响。显微镜观察5,6-羧基荧光素二乙酸酯染料的细胞内运动。虽然单独以1.0或3.5 mW / cm(2)的MMW暴露1 h不会影响GJIC,但是与5 ng / ml TPA处理相结合的MMW暴露可以逆转TPA诱导的GJIC抑制。以1.0 mW / cm(2)的曝光量导致部分逆转,而以3.5 mW / cm(2)的曝光量则导致TPA抑制作用基本上完全逆转。生物电磁学25:1-4,2004。

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